edu
Subject: RE: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our "negative tissue
control".
Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)26
...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, April 30, 2014 8:35 AM
To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our "
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha
M.
Sent: Wednesday, April 30, 2014 6:48 AM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
So this has confused me more.
(w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri
Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls
On
lf Of Terri
Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls
On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must h
On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must have a negative tissue
control, which to quote CAP: "must show no staining of tissues known to
lack the antigen"
Any of the following can serve
I apologize for the delay in responding to your e-mail; I just returned from
the 2014 Applied IHC and Molecular Morphology meeting in Miami where this
subject was discussed (as you might expect).
The answer is, "Yes". The bottom line is that every laboratory running
non-avidin-biotin-based dia
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, February 12, 2014 11:04 AM
To: Cheri Miller
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Controls for IHC
Cheryl
Yes, I
te: Wed, 12 Feb 2014 08:04:43 -0600
> Subject: RE: [Histonet] RE: Negative Controls for IHC
>
> I just stopped using the reagent controls on all our IHC due to the drastic
> cuts in reimbursement. It will be a significant immediate savings for our
> tiny lab.
> Cheryl A. Miller
Tanya'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Controls for IHC
Not too long ago, there was a posting about labs that had discontinued. You
technically don't need with a polymer, non-biotin system. I still do them- for
now. The pathologist never uses them, interna
oelle Weaver MAOM, HTL (ASCP) QIHC
> From: lseb...@uwhealth.org
> To: tanyaabb...@catholichealth.net; histonet@lists.utsouthwestern.edu
> Date: Tue, 11 Feb 2014 19:44:37 +
> CC:
> Subject: [Histonet] RE: Negative Controls for IHC
>
> Hi Tanya,
>
> We have made
Hi Tanya,
We have made the decision to continue running negative reagent control slides.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lis
The "Evidence" should come from your own laboratory. Just because we don't run
"Negative reagent controls" in my laboratory doesn't mean it's appropriate for
you to discontinue their use for your laboratory. Your laboratory Medical
Director needs to make this decision based on what he/she sees
This is the key. What is the evidence based research/studies to support this?
I will continue with negative controls until I am able to get peer reviewed
research to support this.
Besides overkill maybe pricey ($$) at times or not (since it is just slides)
but from what I learned this past we
mw...@wakehealth.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, August 17, 2012 12:36 PM
To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu
Subject:
error in a separate email.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, August 17, 2012 12:36 PM
To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu
Su
For everyone performing immunos are you not running negative controls now with
this new ruling from CAP?
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302 o
We do not run a second negative control. I agree that it wastes reagents and
precious tissue.
Martha Ward
Wake Forest University Baptist Medical Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
I was also taught to run a positive and negative every time you do an
immuno stain, provided there is sufficient tissue. I was trained at AFIP, by
the people that wrote the second AFIP staining manual (the red one). Of
course, if cost is your number one concern.
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon,
Loralee A
Sent: Tuesday, March 01, 2011 1:30 PM
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: negative controls on immunos
We do not run additional n
We do not run additional negatives with each run on the same block. If it was
a different block then yes we would run a negative.
It takes up too many spaces on the machine and uses reagents.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of S
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