The section of skin is too large. It should be no thicker than approximately 3mm. If you need to see the entire piece of skin, you should be able to submit multiple thin sections. A standard 12hr processing schedule works. I am not familiar with zinc formalin, but the standard fixation time for formalin is, minimally 24hrs, therefore I would guess your fixation time is also too short.
Dolores -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shaw, Sharon Sent: Thursday, January 27, 2011 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat Skin Can anybody share their protocol on processing rat skin I seem to be having a problem with it being under processed. I fix the tissue for 11hrs in zinc formalin and then run a 12 hour processing schedule the skin size is 2.5cmx1cm. Thanks, Sharon _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of , or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
