With slides this old you can usually spray the back of the slide with freeze spray and the coverslip will pop off. Then soak in xylene and once any remaining permount is gone, re-coverslip. We did this routinely at AFIP, in the old days.
James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US) Sent: Wednesday, August 27, 2014 8:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide remounting Importance: High Does anyone have a procedure that removes coverslips (w/crystallized permount present) ? The slides are 10+ years old. And is there a process to liquify the crystallized permount and refill w/o removing the coverslip? The mounted slides are 3 x 1's Juan l. Bassett HT ASCP Histotechnician Anatomical Div. National Museum of Health and Medicine 2500 Linden Lane Silver Spring, MD 20910 Office 301-319-3359 Lab 301-319-3328 Mobile 240-505-7247 juan.l.bassett....@mail.mil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
