[Histonet] RE: Uneven Staining

2012-11-28 Thread Debbie Faichney
19:13 To: Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Barry, Thanks for the suggestion. We are generally only able to get about 10-15 sections in a ribbon due to the nature of the tissue, and the pale staining last for 30+ sections at times. We

[Histonet] RE: Uneven Staining

2012-11-28 Thread Lynette Pavelich
2012 19:13 To: Rittman, Barry R; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Barry, Thanks for the suggestion. We are generally only able to get about 10-15 sections in a ribbon due to the nature of the tissue, and the pale staining last for 30+ sections at times

[Histonet] RE: Uneven Staining

2012-11-27 Thread Rittman, Barry R
Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place this on the water bath and then cut the next portion of ribbon then perhaps the

[Histonet] RE: Uneven Staining

2012-11-27 Thread Elizabeth Cameron
PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Uneven Staining Elizabeth as the sections are evenly stained but vary in their staining from slide to slide, one suggestion is that section thickness varies. I know that this is reaching but if you section a ribbon and then place