I always recommend that cytology specimens be collected (or rinsed) in sterile
saline or RPMI. Although fixation in alcohol can preserve some proteins very
nicely, it can also damage others. CytoLyt contains methanol. Interesting
that your Cytopath MDs still use B72.3; most labs have stopped
Well actually it does help because we are trying to eliminate possible causes
and it is nice to know that someone else uses Cytolyt with no problems. It is
just frustrating because we are doing nothing different that I can find.The
next step is to see if the samples are coming from the
We have not used B72.3 for quite awhile now but all of our NGYNs are either
collected in or processed with Cytolyt and we have had no issues. Sorry, I
know this isn't much help.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166