[Histonet] RE: cell block staining issues

2013-11-03 Thread Cartun, Richard
I always recommend that cytology specimens be collected (or rinsed) in sterile saline or RPMI. Although fixation in alcohol can preserve some proteins very nicely, it can also damage others. CytoLyt contains methanol. Interesting that your Cytopath MDs still use B72.3; most labs have stopped

[Histonet] RE: cell block staining issues

2013-10-31 Thread Martha Ward-Pathology
Well actually it does help because we are trying to eliminate possible causes and it is nice to know that someone else uses Cytolyt with no problems. It is just frustrating because we are doing nothing different that I can find.The next step is to see if the samples are coming from the

[Histonet] RE: cell block staining issues

2013-10-31 Thread Tom McNemar
We have not used B72.3 for quite awhile now but all of our NGYNs are either collected in or processed with Cytolyt and we have had no issues. Sorry, I know this isn't much help. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166