Denise We use paraplast and paraplast extra and have not experienced any problems. We just got a new lot of paraplast extra in and so far everything seems ok. I would check to see if it's a particular lot number that you are using.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 7:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu<mailto:dencr...@mit.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet