We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the pre-marked slides. After processing we make a box with a red sharpie around the control tissue so there is no confusion as to which is the patient tissue and which is control.
Sara McCabe, HT (ASCP) DuBois Regional Medical Center ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, July 21, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Assisting with Autopsies (Horn,Hazel) 2. Re: Leica Service Technician (Nicole Tatum) 3. RE: Leica Service Technician (Suresch, Donna L.) 4. Research microtomes (Adrienne Anderson) 5. Re: Research microtomes (Jack Ratliff) 6. Re: Research microtomes (Adrienne Anderson) 7. Re: Research microtomes (Jack Ratliff) 8. embalmers using formaldehyde - NYT story (Bob Richmond) 9. Re: Microtome draft shield (Joseph Saby) 10. Re: (Denise Piontek) 11. Re: Research microtomes (Adrienne Anderson) 12. Re: Research microtomes (Jack Ratliff) 13. Small Slide Scanner (Sheila Haas) 14. Fast Blue stabilization (carpj) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Jul 2011 12:17:48 -0500 From: "Horn,Hazel" <hor...@archildrens.org> Subject: [Histonet] RE: Assisting with Autopsies To: "'Amy Self'" <as...@georgetownhospitalsystem.org>, "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <25a4de08332b19499904459f00aaacb7198b85a...@evs1.archildrens.org> Content-Type: text/plain; charset="us-ascii" We have an autopsy assistant and when he's not busy with autopsies (which is often) he works in our histology lab as an assistant!! Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Autopsy/Histology/Transcription Arkansas Children's Hospital 1 Children's Way Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3155 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Wed, 20 Jul 2011 14:08:10 -0400 (EDT) From: "Nicole Tatum" <nic...@dlcjax.com> Subject: Re: [Histonet] Leica Service Technician To: "Amanda Madden" <amkmad...@gmail.com>, histonet@lists.utsouthwestern.edu Message-ID: <3934.208.62.167.196.1311185290.squir...@webmail.realpages.com> Content-Type: text/plain;charset=iso-8859-1 Amanda, Im not exactly sure which part of your machine is freezing based on your description, but, I have a leica 1510 and it has freezing issues. Everyone I have ever used does this. The bar that hold the specimen chucks ices and freezes over like crazy.. It will frezze the chuck in the bar holder solid. I have to get a hammer and beat it sometimes..lol. So, before I use my machine I wipe 100% alcohol across the bar to de-ice it and the chucks dont get stuck..Do not use so much that you lower your temp. Also, do not get on the oct or the stage because your blocks will not cut and be mush... Just wipe the areas daily with alcohol.. Hope this helps. Nicole Tatum, HT ASCP Hi Histonetters! > > A few months back I emailed regarding a service contract through Leica for > our CM 3050S, and (unfortunately?) we chose not to purchase one. This week > we have had a serious issue with it... the specimen head and arm is > covered > in frost, and the object temperature sensor is reading ## instead of a > temp. > In any case, we called Leica and asked for a service call, but it is > extremely expensive and they couldn't give us an estimate of when they > will > be here because cryostats used for clinical applications have priority > over > those, like ours, that are used for research. Understandable, but > frustrating nonetheless. So my question is: does anyone know of a good, > reputable cryostat service technician (who is authorized by leica, if > possible) that is located in the Boston, MA area? > > Any help would be greatly appreciated! > > Thanks in advance, > Amanda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 3 Date: Wed, 20 Jul 2011 14:16:40 -0400 From: "Suresch, Donna L." <donna_sure...@merck.com> Subject: [Histonet] RE: Leica Service Technician To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2c9a1d9608959940943f357e0a470ff89765a7f...@usctmxp51005.merck.com> Content-Type: text/plain; charset="us-ascii" Hello Amanda, We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662). We have been very happy with their service on our cryostats. Donna L. Suresch Merck Research Laboratories Research Biologist Imaging Research - West Point -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, July 20, 2011 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 92, Issue 27 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Leica Service Technician (Amanda Madden) 2. Controls with patient specimen on same slide (shargr...@urhcs.org) 3. please remove me from the list, thank you! (Robert Cordero) 4. RE: Leica Service Technician (Sherwood, Margaret ) 5. Cat Scratch control (Houston, Ronald) 6. RE: Leica Service Technician (Tom McNemar) 7. RE: Controls with patient specimen on same slide (Tom McNemar) 8. RE: Assisting with Autopsies (Weems, Joyce) 9. Controls with patient specimen on same slide (Nancy Schmitt) ---------------------------------------------------------------------- Message: 1 Date: Wed, 20 Jul 2011 11:32:07 -0400 From: Amanda Madden <amkmad...@gmail.com> Subject: [Histonet] Leica Service Technician To: histonet@lists.utsouthwestern.edu Message-ID: <CAC=+zgXtEK9=c0_nUSddHcSp4_7SDsn2jFxT3kYCJh=nswu...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda ------------------------------ Message: 2 Date: Wed, 20 Jul 2011 10:35:33 -0500 From: shargr...@urhcs.org Subject: [Histonet] Controls with patient specimen on same slide To: histonet@lists.utsouthwestern.edu Message-ID: <ofe0363896.5e031032-on862578d3.005508b0-862578d3.0055a...@urhcs.org> Content-Type: text/plain; charset="us-ascii" In our Lab we put a control on every slide. We keep multiple controls cut and stored in refrigerator. Most are multi- tissue controls. We use the Ventana detection kits and it is not cost efficient to use 2 uses of the detection kit per antibody. Of course there are times that this will not work, then our cost per test goes up, but we cannot charge any more for a control. (Embedded image moved to file: pic32000.jpg) ------------------------------ Message: 3 Date: Wed, 20 Jul 2011 15:37:44 +0000 From: Robert Cordero <robert.cord...@comphealth.com> Subject: [Histonet] please remove me from the list, thank you! To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <0ba36f96367e8a4cbb27e112faa6488a067...@vslcexmbp02.mychg.com> Content-Type: text/plain; charset="us-ascii" ------------------------------ Message: 4 Date: Wed, 20 Jul 2011 11:43:46 -0400 From: "Sherwood, Margaret " <msherw...@partners.org> Subject: RE: [Histonet] Leica Service Technician To: "Amanda Madden" <amkmad...@gmail.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <073ae2bea1c2ba4a8837ab6c4b943d9708db5...@phsxmb30.partners.org> Content-Type: text/plain; charset="us-ascii" Amanda, We have a Microm HM550 cryostat and have used Brian J. Hurley (New England Biomedical Services). He is an independent service engineer. He's very good. In fact ThermoFisher uses him if a service visit is needed. 781-331-8642 617-774-7368 (cell) Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ------------------------------ Message: 5 Date: Wed, 20 Jul 2011 15:55:50 +0000 From: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> Subject: [Histonet] Cat Scratch control To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu>, "ih...@googlegroups.com" <ih...@googlegroups.com> Message-ID: <e5d0cd2352e46545a0c0ebe308ccce530bc...@l1perdwxmb01.childrensroot.net> Content-Type: text/plain; charset="us-ascii" Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good. Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> www.NationwideChildrens.org<http://www.NationwideChildrens.org> "One person with passion is better than forty people merely interested." ~ E.M. Forster ----------------------------------------- Confidentiality Notice: The following mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. The recipient is responsible to maintain the confidentiality of this information and to use the information only for authorized purposes. If you are not the intended recipient (or authorized to receive information for the intended recipient), you are hereby notified that any review, use, disclosure, distribution, copying, printing, or action taken in reliance on the contents of this e-mail is strictly prohibited. If you have received this communication in error, please notify us immediately by reply e-mail and destroy all copies of the original message. Thank you. ------------------------------ Message: 6 Date: Wed, 20 Jul 2011 12:26:11 -0400 From: Tom McNemar <tmcne...@lmhealth.org> Subject: RE: [Histonet] Leica Service Technician To: 'Amanda Madden' <amkmad...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e9a90e28259d2f4e84308c5e8ea8f7b4381967a...@lmhs-exchange.lmhealth.org> Content-Type: text/plain; charset="us-ascii" It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Madden Sent: Wednesday, July 20, 2011 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica Service Technician Hi Histonetters! A few months back I emailed regarding a service contract through Leica for our CM 3050S, and (unfortunately?) we chose not to purchase one. This week we have had a serious issue with it... the specimen head and arm is covered in frost, and the object temperature sensor is reading ## instead of a temp. In any case, we called Leica and asked for a service call, but it is extremely expensive and they couldn't give us an estimate of when they will be here because cryostats used for clinical applications have priority over those, like ours, that are used for research. Understandable, but frustrating nonetheless. So my question is: does anyone know of a good, reputable cryostat service technician (who is authorized by leica, if possible) that is located in the Boston, MA area? Any help would be greatly appreciated! Thanks in advance, Amanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 7 Date: Wed, 20 Jul 2011 12:30:06 -0400 From: Tom McNemar <tmcne...@lmhealth.org> Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Rathborne, Toni'" <trathbo...@somerset-healthcare.com>, 'Richard Cartun' <rcar...@harthosp.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e9a90e28259d2f4e84308c5e8ea8f7b4381967a...@lmhs-exchange.lmhealth.org> Content-Type: text/plain; charset="us-ascii" Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Wednesday, July 20, 2011 9:19 AM To: 'Richard Cartun'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Controls with patient specimen on same slide All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? -----Original Message----- From: Richard Cartun [mailto:rcar...@harthosp.org] Sent: Tuesday, July 19, 2011 6:40 PM To: histonet@lists.utsouthwestern.edu; Rathborne, Toni Subject: Re: [Histonet] Controls with patient specimen on same slide We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Rathborne, Toni" <trathbo...@somerset-healthcare.com> 7/19/2011 >>> 3:27 PM >>> Hi, I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not? Thanks, Toni CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This message was secured by ZixCorp(R). CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ------------------------------ Message: 8 Date: Wed, 20 Jul 2011 12:53:21 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] RE: Assisting with Autopsies To: Amy Self <as...@georgetownhospitalsystem.org>, "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164082122a...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years. Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Self Sent: Tuesday, July 19, 2011 15:12 To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Assisting with Autopsies Hello All, We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll. Does your facility use histotechs or a diener to assist with the autopsy? Thanks in advance for all of your help, Amy Amy Self Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 9 Date: Wed, 20 Jul 2011 16:59:53 +0000 From: Nancy Schmitt <nancy_schm...@pa-ucl.com> Subject: [Histonet] Controls with patient specimen on same slide To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <906b4da90ed1db4db6c7e94d7cee6c36791...@peitha.wad.pa-ucl.com> Content-Type: text/plain; charset="us-ascii" We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case. Nancy Schmitt HT, MLT(ASCP) United Clinical Laboratories ----------------------------------------- Message: 12 Date: Wed, 20 Jul 2011 13:18:52 +0000 From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> Subject: RE: [Histonet] Controls with patient specimen on same slide To: "'Richard Cartun'" <rcar...@harthosp.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3ad061fe740d464fac7bf6b5cfb75707031...@smcmail01.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" All of these responses are great. So here's a follow up question. Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides? NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 27 **************************************** Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 4 Date: Wed, 20 Jul 2011 12:48:14 -0700 (PDT) From: Adrienne Anderson <rennie1...@yahoo.com> Subject: [Histonet] Research microtomes To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Cc: "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <1311191294.91032.yahoomail...@web59614.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hello Histo-land, I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated! Thanks, Adrienne ------------------------------ Message: 5 Date: Wed, 20 Jul 2011 16:02:19 -0500 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson <rennie1...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <blu0-smtp96debefcd953a5410dd7cae...@phx.gbl> Content-Type: text/plain; charset="us-ascii" What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. > I've only had clinical experience, so I don't know of any such microtome. Any > advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Wed, 20 Jul 2011 14:57:48 -0700 (PDT) From: Adrienne Anderson <rennie1...@yahoo.com> Subject: Re: [Histonet] Research microtomes To: Jack Ratliff <ratliffj...@hotmail.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <1311199068.60287.yahoomail...@web59611.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Jack, We're trying to cut just plain old FFPE blocks. ________________________________ From: Jack Ratliff <ratliffj...@hotmail.com> To: Adrienne Anderson <rennie1...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" <mlosbo...@gmail.com> Sent: Wednesday, July 20, 2011 5:02 PM Subject: Re: [Histonet] Research microtomes What type of specimen are you trying to cut? What embedding media are you using? Jack On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > Hello Histo-land, > > I'm trying to find a microtome that can cut from 50-2000 micron sections. > I've only had clinical experience, so I don't know of any such microtome. Any > advice would be appreciated! > > Thanks, > Adrienne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 7 Date: Wed, 20 Jul 2011 17:13:43 -0500 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson <rennie1...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <blu0-smtp1328efabcd05a07be45e5d1ae...@phx.gbl> Content-Type: text/plain; charset="us-ascii" Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > Hi Jack, > > We're trying to cut just plain old FFPE blocks. > > From: Jack Ratliff <ratliffj...@hotmail.com> > To: Adrienne Anderson <rennie1...@yahoo.com> > Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; > "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" > <mlosbo...@gmail.com> > Sent: Wednesday, July 20, 2011 5:02 PM > Subject: Re: [Histonet] Research microtomes > > What type of specimen are you trying to cut? What embedding media are you > using? > > Jack > > > > On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > > > Hello Histo-land, > > > > I'm trying to find a microtome that can cut from 50-2000 micron sections. > > I've only had clinical experience, so I don't know of any such microtome. > > Any advice would be appreciated! > > > > Thanks, > > Adrienne > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ Message: 8 Date: Wed, 20 Jul 2011 18:45:19 -0400 From: Bob Richmond <rsrichm...@gmail.com> Subject: [Histonet] embalmers using formaldehyde - NYT story To: path...@mailman.srv.ualberta.ca, "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <caoksrh7oivhwtgytkj0q-vhs41lddp04hy+zocchcku4xdv...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 This New York Times story about the use of formaldehyde by embalmers is worth reading by pathologists and histotechnologists. http://www.nytimes.com/2011/07/21/business/despite-cancer-risk-embalmers-stay-with-formaldehyde.html?_r=1&hp Seems like they've basically got the right idea. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 9 Date: Wed, 20 Jul 2011 15:45:47 -0700 (PDT) From: Joseph Saby <saby_josep...@yahoo.com> Subject: Re: [Histonet] Microtome draft shield To: "Rathborne, Toni" <trathbo...@somerset-healthcare.com>, 'Keri Colwell' <keri.colw...@inspection.gc.ca>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1311201947.45523.yahoomail...@web114401.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would suggest that you look inthe local yellow pages for people who work with plexiglass.? This is a re;atively inexpensive medium.? You can design what you need and have your draft shields built to order. ? Joe Saby NAMSA From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> To: 'Keri Colwell' <keri.colw...@inspection.gc.ca>; "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, July 19, 2011 9:11 AM Subject: RE: [Histonet] Microtome draft shield Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Keri Colwell Sent: Monday, July 18, 2011 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome draft shield Hi Everyone, I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways.? We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons. Anyone have any suggestions as to who might sell such a thing? Thanks in advance! Keri Colwell Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640? | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colw...@inspection.gc.ca Telephone | T?l?phone:? 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee.? The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 21 Jul 2011 09:11:33 -0400 From: Denise Piontek <dbpion...@hotmail.com> Subject: [Histonet] Re: To: <camille_di...@bio-rad.com>, <cdi...@gmail.com>, <canvaspeo...@email.canvaspeople.com>, <dpant...@hrecruiters.com>, <deedra...@yahoo.com>, <histonet@lists.utsouthwestern.edu>, <i...@littlepeopleplayhouse.com> Message-ID: <col112-w55a0b140d232a1b964abf0ab...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Sexual problems are to be solved... http://ravera.todouy.com/bestsite.php?opage=20f0 ------------------------------ Message: 11 Date: Thu, 21 Jul 2011 07:49:22 -0700 (PDT) From: Adrienne Anderson <rennie1...@yahoo.com> Subject: Re: [Histonet] Research microtomes To: Jack Ratliff <ratliffj...@hotmail.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <1311259762.22661.yahoomail...@web59603.mail.ac4.yahoo.com> Content-Type: text/plain; charset=us-ascii This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing: "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth. Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns." Thanks, Adrienne ________________________________ From: Jack Ratliff <ratliffj...@hotmail.com> To: Adrienne Anderson <rennie1...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" <mlosbo...@gmail.com> Sent: Wednesday, July 20, 2011 6:13 PM Subject: Re: [Histonet] Research microtomes Couple more questions. :) What is the tissue and the dimensions of the specimen? On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: Hi Jack, > >We're trying to cut just plain old FFPE blocks. > > > >________________________________ >From: Jack Ratliff <ratliffj...@hotmail.com> >To: Adrienne Anderson <rennie1...@yahoo.com> >Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; >"bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" ><mlosbo...@gmail.com> >Sent: Wednesday, July 20, 2011 5:02 PM >Subject: Re: [Histonet] Research microtomes > >What type of specimen are you trying to cut? What embedding media are you >using? > >Jack > > > >On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > >> Hello Histo-land, >> >> I'm trying to find a microtome that can cut from 50-2000 micron sections. >> I've only had clinical experience, so I don't know of any such microtome. >> Any advice would be appreciated! >> >> Thanks, >> Adrienne >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ Message: 12 Date: Thu, 21 Jul 2011 10:07:09 -0500 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: Re: [Histonet] Research microtomes To: Adrienne Anderson <rennie1...@yahoo.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>, "mlosbo...@gmail.com" <mlosbo...@gmail.com> Message-ID: <blu0-smtp12232da09f9cb6ea8cfd67dae...@phx.gbl> Content-Type: text/plain; charset="us-ascii" Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975. Best Regards, Jack On Jul 21, 2011, at 9:49 AM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > This post is actually on behalf of some interns that are working at my > company over the summer. Their emails are included on this post, so I'm going > to turn it over to them:) But here is their description of what they're doing: > > "In answer to Jack's questions (I thought about replying myself, but didn't > want to confuse anyone), we want to make a CEMA array (cutting edge matrix > assembly array), which could be done with a variety of tissue types. The step > that calls for the special microtome is when you cut the original blocks from > the hospital -- meaning variable tissue depth. > > Though the article mentioned a microtome that could cut 50-2000, we are most > interested in the range around 50-150 microns." > > Thanks, > Adrienne > > From: Jack Ratliff <ratliffj...@hotmail.com> > To: Adrienne Anderson <rennie1...@yahoo.com> > Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; > "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" > <mlosbo...@gmail.com> > Sent: Wednesday, July 20, 2011 6:13 PM > Subject: Re: [Histonet] Research microtomes > > Couple more questions. :) What is the tissue and the dimensions of the > specimen? > > > > On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: > >> Hi Jack, >> >> We're trying to cut just plain old FFPE blocks. >> >> From: Jack Ratliff <ratliffj...@hotmail.com> >> To: Adrienne Anderson <rennie1...@yahoo.com> >> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>; >> "bush...@rose-hulman.edu" <bush...@rose-hulman.edu>; "mlosbo...@gmail.com" >> <mlosbo...@gmail.com> >> Sent: Wednesday, July 20, 2011 5:02 PM >> Subject: Re: [Histonet] Research microtomes >> >> What type of specimen are you trying to cut? What embedding media are you >> using? >> >> Jack >> >> >> >> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1...@yahoo.com> wrote: >> >> > Hello Histo-land, >> > >> > I'm trying to find a microtome that can cut from 50-2000 micron sections. >> > I've only had clinical experience, so I don't know of any such microtome. >> > Any advice would be appreciated! >> > >> > Thanks, >> > Adrienne >> > _______________________________________________ >> > Histonet mailing list >> > Histonet@lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> > > ------------------------------ Message: 13 Date: Thu, 21 Jul 2011 08:34:10 -0700 (PDT) From: Sheila Haas <micropathl...@yahoo.com> Subject: [Histonet] Small Slide Scanner To: histonet@lists.utsouthwestern.edu Message-ID: <1311262450.88288.yahoomai...@web161715.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi all. Is anyone out there using one of the small, low volume slide scanners for use in?frozen sections? We have?looked at a couple of the larger scanners for other purposes but?wanted to know how the smaller ones would?work for our pathologists to?review frozen slides from off-site facilities. Of course, a PA would gross?the specimen at the facility,?cut the slides and?then load them into the scanner. Any thoughts? ? Sheila Haas Laboratory Supervisor MicroPath Laboratories, Inc. ------------------------------ Message: 14 Date: Thu, 21 Jul 2011 12:36:59 -0400 From: carpj <ca...@wadsworth.org> Subject: [Histonet] Fast Blue stabilization To: histonet@lists.utsouthwestern.edu Message-ID: <a37574e6-6cb6-4f02-9955-a59c84837...@wadsworth.org> Content-Type: text/plain; charset=iso-8859-1 Hi List, We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-?m) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing? Jonathan Carp, Ph.D. Wadsworth Center New York State Dept. Health Albany, NY IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 28 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet