John Have you tried paraffin? I know that we have been able to section some plastic devices with paraffin sections, it may be worth a try. Looks like the device you are working with is compatable with xylene, we have found in some cases that we need to use a xylene subsititute.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Baker, John [bak...@med.umich.edu] Sent: Thursday, February 07, 2013 8:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding problem I had to sign up again for the Histonet so I am not sure if this question went out so will resend. thanks Hello Histonetters, We are trying to embed a polycarbonate device with soft tissue attached to look at the implant interface. The problem is that with several standard protocols for pmma processing the clearing agent (methyl salicylate or xylene) and the methacrylate monomer dissolves the polycarbonate. Does anyone have any experience trying process such a thing, an embedding media (pmma, OCT for cryo, etc.), and then a method of sectioning it keeping the interface intact? Thanks in advance for any suggestions! John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
