We have not had any problem with fluorescence with our her 2 FISH or any complaints from the Cytogenetics labs which run many other types of FISH. We use eosin in our processors. The eosin will bleed out when you deparaffinize the slides.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kuhnla, Melissa [melissa.kuh...@chsli.org] Sent: Thursday, November 04, 2010 9:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eosin as tissue dye I know using eosin as a tissue dye during processing has been a recent discussion. My lab has been experiencing issue with the cobalt blue we use currently. At embedding, small biopsies are not seen on blue sponges, white sponges turn blue. Biopsies that are wrapped are not turning blue, the paper is not allowing the blue thru. Eosin has come up as a suggestion. I know it is known to fluoresce. What is everyone's experience with this?? The only FISH test we are running is Her2 by pathvysion. What recipe are you all using?? On the processor?? Thank you so much. Melissa The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet