Of Terri
Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls
On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must have
(w)
304-685-9307 (c)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri
Braud
Sent: Tuesday, April 29, 2014 1:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Negative Controls
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha
M.
Sent: Wednesday, April 30, 2014 6:48 AM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
So this has confused me more. So before you would
...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Wednesday, April 30, 2014 8:35 AM
To: 'Campbell, Tasha M.'; Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our negative tissue
: [Histonet] Re: Negative Controls
Tasha,
We use the negative elements within our patient sample as our negative tissue
control.
Linda A. Sebree
University of Wisconsin Hospital Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message
On Message 7 - Negative Controls
While it is true that if you run polymers, you no longer have to run a
negative reagent control, HOWEVER, you still must have a negative tissue
control, which to quote CAP: must show no staining of tissues known to
lack the antigen
Any of the following can serve
I apologize for the delay in responding to your e-mail; I just returned from
the 2014 Applied IHC and Molecular Morphology meeting in Miami where this
subject was discussed (as you might expect).
The answer is, Yes. The bottom line is that every laboratory running
non-avidin-biotin-based
08:04:43 -0600
Subject: RE: [Histonet] RE: Negative Controls for IHC
I just stopped using the reagent controls on all our IHC due to the drastic
cuts in reimbursement. It will be a significant immediate savings for our
tiny lab.
Cheryl A. Miller HT ASCP cm
Histology Supervisor
Hygiene
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, February 12, 2014 11:04 AM
To: Cheri Miller
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Controls for IHC
Cheryl
Yes, I
Hi Tanya,
We have made the decision to continue running negative reagent control slides.
Linda A. Sebree
University of Wisconsin Hospital Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From:
Weaver MAOM, HTL (ASCP) QIHC
From: lseb...@uwhealth.org
To: tanyaabb...@catholichealth.net; histonet@lists.utsouthwestern.edu
Date: Tue, 11 Feb 2014 19:44:37 +
CC:
Subject: [Histonet] RE: Negative Controls for IHC
Hi Tanya,
We have made the decision to continue running negative
The Evidence should come from your own laboratory. Just because we don't run
Negative reagent controls in my laboratory doesn't mean it's appropriate for
you to discontinue their use for your laboratory. Your laboratory Medical
Director needs to make this decision based on what he/she sees
This is the key. What is the evidence based research/studies to support this?
I will continue with negative controls until I am able to get peer reviewed
research to support this.
Besides overkill maybe pricey ($$) at times or not (since it is just slides)
but from what I learned this past
For everyone performing immunos are you not running negative controls now with
this new ruling from CAP?
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1302
in a separate email.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, August 17, 2012 12:36 PM
To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE
...@wakehealth.edu
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Friday, August 17, 2012 12:36 PM
To: 'Romundstad, Pamela K'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McMahon,
Loralee A
Sent: Tuesday, March 01, 2011 1:30 PM
To: Diana McCaig; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: negative controls on immunos
We do not run additional negatives with each run
I was also taught to run a positive and negative every time you do an
immuno stain, provided there is sufficient tissue. I was trained at AFIP, by
the people that wrote the second AFIP staining manual (the red one). Of
course, if cost is your number one concern.
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