another piece of fixed tissue , process it with standard paraffin
processing protocol.
Dr. Amita Dubey
PCSED,TRC
From:
John Kiernan
To:
"Thomas, Nancy"
Cc:
"histonet@lists.utsouthwestern.edu" ,
'An Eerdekens'
Date:
02/02/11 12:28 PM
Subject:
Re: [Histonet] RE:
You can't go from 70% alcohol into paraffin without passing through 100%
alcohol and then a clearing agent (liquid miscible with 100% alcohol and with
melted wax). Xylene is a commonly used clearing agent. Your times in 50% and
70% alcohol are much longer than necessary. Even for a whale's hypot
Hi An,
You did not mention a clearing step (xylene, or xylene substitute) between your
alcohol and paraffin steps. Maybe you just forgot to write it, but if you
actually missed this step, your samples would be improperly processed leading
to the problems you have.
Nancy Thomas
Stowers Institut
