[Histonet] RE: rat muscle fixation

2012-06-19 Thread Pixley, Sarah (pixleysk)
Thank you Dr. Wright! We have full IACUC approval for perfusion and for all our procedures, and we have done a lot of paraformaldehyde perfusion fixation. It is just that for this study, we would like to avoid it, since it might interfere with other aspects of the experiment. So, we were wonder

[Histonet] Re: rat muscle fixation

2012-06-19 Thread David A. Wright
Hello Sarah and Histonet You get wonderful formalin fixation of all rat tissues by transcardial perfusion - and no blood cells. You don't say whether you need a specific muscle and if it needs to be from a specific rat. Before you get into the bother of perfusing (& IACUC approval) you might w