We retain our empty containers for 2 weeks post-final report. D. Goodwin SJGI Marlton, NJ
________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, June 23, 2010 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 79, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Breezy lab/flyaway ribbons! (R J VAZQUEZ) 2. slim jims (Perry, Margaret) 3. RE: Breezy lab/flyaway ribbons! (Gill, Caula A.) 4. Re: Autofluorescence in murine white adipose tissue cryosections (Johnson, Teri) 5. Re: slim jims (V. Neubert) 6. X-gal staining troubleshooting (Johnson, Teri) 7. Glut1 antibody (Martha Ward) 8. RE: Breezy lab/flyaway ribbons! (Sebree Linda A) 9. Re: slim jims (Emily Sours) 10. IHC for Legionella? (Harrison, Sandra C.) 11. (no subject) (Hartz, Rhonda SktnHR) 12. Re: IHC for Legionella? (Richard Cartun) 13. Re: Help! In need of positive Gram Control (Jay Lundgren) 14. Re: (no subject) (Jennifer MacDonald) 15. Oven for paraffin slide drying (Mia Woodruff) 16. Re: Help! In need of positive Gram Control (Jackie M O'Connor) (Robert Richmond) 17. Re: Oven for paraffin slide drying (Emily Sours) 18. Re: Re: Amyloidosis control tissue (Komal Gada) 19. RE: Help! In need of positive Gram Control (connie grubaugh) 20. RE: New CAP question ANP.22760 (jmye...@aol.com) 21. RE: Help! In need of positive Gram Control (susan.wal...@hcahealthcare.com) 22. RE: Glut1 antibody (Hoekert, W.E.J.) 23. Retaining empty jars (mtitf...@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 22 Jun 2010 11:07:32 -0700 From: R J VAZQUEZ <sweethin...@msn.com> Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: <histot...@imagesbyhopper.com>, <trathbo...@somerset-healthcare.com>, <jcbrit...@cheshire-med.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <snt121-w529a2ce59d5619e7bfcc8cb8...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histot...@imagesbyhopper.com > To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet the > brush they use to take the ribbon off the knife and 2) to roll/curl the > ribbon over the damp end of the brush and 3) hover our hand over the top of > the ribbon while moving it (to reduce the breeze) from the microtome to the > waterbath. > > I learned this technique when I first started in Histology, as we had a > choice, a HOT room with no AC and no wind, or learn how to deal with the > breeze and don't sweat! I opted for dealing with the wind. Now, it's > second nature and it matters not whether there is wind or not, I still use > the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, > Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The ones > we have are made of stainless, are slightly larger than the vent, and are > suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any attachments, > is for the sole use of the intended recipients and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by electronic mail and destroy all copies of the original > message. _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error by > e-mail or you may call Somerset Medical Center's computer Help Desk at > 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: 06/22/10 > 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 22 Jun 2010 13:47:33 -0500 From: "Perry, Margaret" <margaret.pe...@sdstate.edu> Subject: [Histonet] slim jims To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fca5ef47f9bc694cbb4c58fea04219634dbdb8d...@sdsu-mbx.jacks.local> Content-Type: text/plain; charset="us-ascii" Do you put the slim jims in formalin and then process them or just put them in the processor? Margaret ------------------------------ Message: 3 Date: Tue, 22 Jun 2010 15:41:41 -0400 From: "Gill, Caula A." <cg...@marylandgeneral.org> Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: "R J VAZQUEZ" <sweethin...@msn.com>, <histot...@imagesbyhopper.com>, <trathbo...@somerset-healthcare.com>, <jcbrit...@cheshire-med.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <087a9911bbafde4b8151cb148586e2c23a9...@mdgen-exch1.marylandgeneral.org> Content-Type: text/plain; charset="us-ascii" Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histot...@imagesbyhopper.com; trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histot...@imagesbyhopper.com > To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 22 Jun 2010 14:52:37 -0500 From: "Johnson, Teri" <t...@stowers.org> Subject: [Histonet] Re: Autofluorescence in murine white adipose tissue cryosections To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec130875d...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Dear Frank, Good luck with ridding your samples of autofluorescence. Fat is always very brightly fluorescent and I suspect it might even be so without any aldehyde fixation. First I wondered how successful you'd be using Sudan Black B, knowing it is a fat stain, and knowing that it's been published for this purpose. So you might try just that and see how that works. In addition you could try using a combination of UV irradiation and the Sudan Black B and see if the combination works for you. They use paraffin sections in this reference, but it might work as well with cryosections. http://www.microscopyu.com/references/pdfs/Viegas_etal_Eur_J_Histochem-51-59-2007.pdf Good luck, and do report back if you find a solution to this dilemma! Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 5 Date: Tue, 22 Jun 2010 21:58:39 +0200 From: "V. Neubert" <histonet.nos...@vneubert.com> Subject: Re: [Histonet] slim jims To: histonet@lists.utsouthwestern.edu Message-ID: <4c2115ef.7030...@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 I just found "a partially eaten Slim Jim snack" picture on wikipedia... Is it Friday again? :-> > Do you put the slim jims in formalin and then process them or just put them > in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Tue, 22 Jun 2010 15:02:44 -0500 From: "Johnson, Teri" <t...@stowers.org> Subject: [Histonet] X-gal staining troubleshooting To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec130875d...@exchmb-02.stowers-institute.org> Content-Type: text/plain; charset="us-ascii" Hi all, I have a question about whole mount x-gal staining. Do any of you have experience with your samples turning brown after incubation with the x-gal? If yes, do you know what causes this and how to keep it from happening? Thanks in advance, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 7 Date: Tue, 22 Jun 2010 16:10:12 -0400 From: "Martha Ward" <mw...@wfubmc.edu> Subject: [Histonet] Glut1 antibody To: <histonet@lists.utsouthwestern.edu> Message-ID: <61135f0455d33347b5aae209b903a30433deb...@exchvs2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 ------------------------------ Message: 8 Date: Tue, 22 Jun 2010 15:28:50 -0500 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: RE: [Histonet] Breezy lab/flyaway ribbons! To: "Gill, Caula A." <cg...@marylandgeneral.org>, "R J VAZQUEZ" <sweethin...@msn.com>, <histot...@imagesbyhopper.com>, <trathbo...@somerset-healthcare.com>, <jcbrit...@cheshire-med.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <8c023b4ab999614ba4791baeb26e2738399...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I do that too using either a wet brush or applicator stick but sometimes the breeze is more like a gale force wind breaking my ribbon apart to then adhere to everything but the surface of my waterbath! Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gill, Caula A. Sent: Tuesday, June 22, 2010 2:42 PM To: R J VAZQUEZ; histot...@imagesbyhopper.com; trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! Ditto, I use a small brush that I got from the craft store and prior to picking up the ribbon I wet the brush and slip it under my ribbon end closest to the blade and presto no flyaways. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of R J VAZQUEZ Sent: Tuesday, June 22, 2010 2:08 PM To: histot...@imagesbyhopper.com; trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Breezy lab/flyaway ribbons! When I was doing histology, I would use a flexible end of a brush to roll the ribbon end closest to the blade and that would secure it beautifully and no fly aways. Robyn Vazquez > From: histot...@imagesbyhopper.com > To: trathbo...@somerset-healthcare.com; jcbrit...@cheshire-med.com; > histonet@lists.utsouthwestern.edu > Date: Tue, 22 Jun 2010 11:10:29 -0400 > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > CC: > > We have a similar situation, but I have shown my techs how to 1) wet > the brush they use to take the ribbon off the knife and 2) to > roll/curl the ribbon over the damp end of the brush and 3) hover our > hand over the top of the ribbon while moving it (to reduce the breeze) > from the microtome to the waterbath. > > I learned this technique when I first started in Histology, as we had > a choice, a HOT room with no AC and no wind, or learn how to deal with > the breeze and don't sweat! I opted for dealing with the wind. Now, > it's second nature and it matters not whether there is wind or not, I > still use the same motion for tranfer of the ribbon! > > Good luck with your situation. :o) > > Michelle > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > Rathborne, Toni > Sent: Tuesday, June 22, 2010 8:50 AM > To: Josie Britton; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Breezy lab/flyaway ribbons! > > > For the ceiling vents, you could ask to have deflectors installed. The > ones we have are made of stainless, are slightly larger than the vent, > and are suspended about 6"-8" below the opening. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Breezy lab/flyaway ribbons! > > > We have been having trouble with big breeze blowing our precious > ribbons > > out of our hands while cutting. We would like a door on the Histology > > lab to cut down on the breeze of people walking by through the hall. > > Our facilities want to find out what other people are doing to stop > this > > problem. We also have air ducts blowing down from above, which is not > > helping the problem. We would like as many labs solutions as possible. > > Our facilities have come up with all these crazy barriers that we > would > > have to move to walk around when we need to put our racks on the > > stainer, answer timers, print more slides, use the oven, etc... > > > > > > > > Any input would be appreciated! > > > > > > > > Breezy girls, > > > > > > > > Josie Britton and Chris Braaten > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail > and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical > Center and are intended only for the addressee. The information > contained in this message is confidential and may contain privileged, > confidential, proprietary and/or trade secret information entitled to > protection and/or exemption from disclosure under applicable law. > Unauthorized forwarding, printing, copying, distribution, or use of > such information is strictly prohibited and may be unlawful. If you > are not the addressee, please promptly delete this message and notify > the sender of the delivery error by e-mail or you may call Somerset > Medical Center's computer Help Desk at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, event > listings, health information and more. > No virus found in this incoming message. > Checked by AVG - www.avg.com > Version: 8.5.439 / Virus Database: 271.1.1/2950 - Release Date: > 06/22/10 06:36:00 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 22 Jun 2010 16:50:06 -0400 From: Emily Sours <talulahg...@gmail.com> Subject: Re: [Histonet] slim jims To: "Perry, Margaret" <margaret.pe...@sdstate.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <aanlktilnr8o7jxqa1olrlerhgn2tp_4gvrzfzxodo...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 if by "processor" you mean "my mouth", yes. the formalin is well, just a formality. HA! Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 2:47 PM, Perry, Margaret <margaret.pe...@sdstate.edu > wrote: > Do you put the slim jims in formalin and then process them or just put them > in the processor? > Margaret > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 10 Date: Tue, 22 Jun 2010 16:21:51 -0500 From: "Harrison, Sandra C." <sandra.harris...@va.gov> Subject: [Histonet] IHC for Legionella? To: <histonet@lists.utsouthwestern.edu> Message-ID: <db425e28065da14faa75a282959620af04397...@vhav23msga2.v23.med.va.gov> Content-Type: text/plain; charset="us-ascii" Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 ------------------------------ Message: 11 Date: Tue, 22 Jun 2010 15:55:07 -0600 From: "Hartz, Rhonda SktnHR" <rhonda.ha...@saskatoonhealthregion.ca> Subject: [Histonet] (no subject) To: <histonet@lists.utsouthwestern.edu> Message-ID: <b7f15445a710ba4fa7b48f2c55134ad404928...@lou.sktnhr.ca> Content-Type: text/plain; charset="us-ascii" Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.ha...@saskatoonhealthregion.ca ------------------------------ Message: 12 Date: Tue, 22 Jun 2010 18:31:49 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: Re: [Histonet] IHC for Legionella? To: <histonet@lists.utsouthwestern.edu>, "Sandra C. Harrison" <sandra.harris...@va.gov> Message-ID: <4c210194.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII I offer IHC for Legionella in my laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Harrison, Sandra C." <sandra.harris...@va.gov> 6/22/2010 5:21 PM >>> Does anyone know of an IHC antibody for Legionella? Sandy C. Harrison, HTL (ASCP) Histology Supervisor Minneapolis VA 612-467-2449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 22 Jun 2010 17:52:34 -0500 From: Jay Lundgren <jaylundg...@gmail.com> Subject: Re: [Histonet] Help! In need of positive Gram Control To: dianar...@aol.com Cc: histonet@lists.utsouthwestern.edu Message-ID: <aanlktim6sji00siz3drxplt6nflubjakzxm9hm0zn...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Slim Jim works great. This is what we used to use at AFIP. Just slice it and process it the same as your regular tissue. Jay A. Lundgren, M.S., HTL (ASCP) Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Tue, 22 Jun 2010 15:57:21 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: Re: [Histonet] (no subject) To: "Hartz, Rhonda SktnHR" <rhonda.ha...@saskatoonhealthregion.ca> Cc: histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: <of57dd29aa.c3c56478-on8825774a.007e0c27-8825774a.007e2...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" We used to keep them for a week in a bag. If there were no problems at the end of the week we disposed of them "Hartz, Rhonda SktnHR" <rhonda.ha...@saskatoonhealthregion.ca> Sent by: histonet-boun...@lists.utsouthwestern.edu 06/22/2010 03:24 PM To <histonet@lists.utsouthwestern.edu> cc Subject [Histonet] (no subject) Hi. This is my first time, so I apologize if I am not clear enough. We have had a request from one of our pathologists to retain empty specimen containers after grossing is complete. Is anyone aware of any recommendations, or does anyone out there retain their empty specimen containers? Rhonda Hartz Technologist Supervisor Anatomic Pathology Division Saskatoon Health Region (306) 655-8197 rhonda.ha...@saskatoonhealthregion.ca _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 23 Jun 2010 09:06:14 +1000 From: Mia Woodruff <mia.woodr...@qut.edu.au> Subject: [Histonet] Oven for paraffin slide drying To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e359f813fba517409a67c9d78d3da33d1cde8e0...@qutexmbx02.qut.edu.au> Content-Type: text/plain; charset="us-ascii" Hello all, I need to purchase an oven for drying slides, on a very limited budget. I came across a "food dehydrator" which looks like an oven and has drawers which are the perfect size for the slide holders to fit into and it can reach a temperature of 60C - and it costs about 1/8 of the price of a normal oven. Can anyone foresee any issues in me using this for drying paraffin slides? As far as I can tell there is a low air flow inside and the temp can be set up to 60 degrees so it seems perfect? Any comments? Thanks Mia ------------------------------ Message: 16 Date: Tue, 22 Jun 2010 19:34:09 -0400 From: Robert Richmond <rsrichm...@gmail.com> Subject: [Histonet] Re: Help! In need of positive Gram Control (Jackie M O'Connor) To: histonet@lists.utsouthwestern.edu Message-ID: <aanlktill6obernspunwb2_wmsnk1xyaahm2gsuun7...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I agree - acute appendicitis with rupture and an abscess provides an abundance of gram positive and gram negative bacteria, and such cases are common. I think that actual clinical tissue is better than controls faked with reference organisms. Tissue gram stains are greatly overrated. They don't work nearly as well as smears do. If you want to see bacteria in tissue sections, stain them with Giemsa or toluidine blue. Bob Richmond Samurai Pathologist Knoxville TN ------------------------------ Message: 17 Date: Tue, 22 Jun 2010 20:16:41 -0400 From: Emily Sours <talulahg...@gmail.com> Subject: Re: [Histonet] Oven for paraffin slide drying To: Mia Woodruff <mia.woodr...@qut.edu.au>, histonet@lists.utsouthwestern.edu Message-ID: <aanlktim_ybupuqezvgfwwe_qybhtj6oh2ivc5fhll...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Do slide warmers cost too much? I don't know how much they cost, though, maybe an oven is cheaper. Emily Towns are like people. Old ones often have character, the new ones are interchangeable. --Wallace Stegner, Angle of Repose On Tue, Jun 22, 2010 at 7:06 PM, Mia Woodruff <mia.woodr...@qut.edu.au> wrote: > Hello all, > > I need to purchase an oven for drying slides, on a very limited budget. I > came across a "food dehydrator" which looks like an oven and has drawers > which are the perfect size for the slide holders to fit into and it can reach > a temperature of 60C - and it costs about 1/8 of the price of a normal oven. > Can anyone foresee any issues in me using this for drying paraffin slides? As > far as I can tell there is a low air flow inside and the temp can be set up > to 60 degrees so it seems perfect? Any comments? > > Thanks > Mia > > > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Tue, 22 Jun 2010 21:01:14 -0400 From: Komal Gada <kjg...@gmail.com> Subject: Re: [Histonet] Re: Amyloidosis control tissue To: Robert Richmond <rsrichm...@gmail.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <aanlktinrmi6n2yhhijwg8ub3fkjmvxwzvolugm3jq...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 American mastertech On Tue, Jun 22, 2010 at 12:19 PM, Robert Richmond <rsrichm...@gmail.com>wrote: > It's always been difficult to get amyloid control tissue. Amyloidosis > is a rather rare disease in the USA, and some cases simply do not > stain well. > > I've asked this question on Histonet several times and no one has ever > responded: Amyloidosis (of the AA type I think) is rather easily > produced in mice and other small experimental animals. Why can't this > tissue be available as an amyloid control? > > Seems that some enterprising person could make a little money this way. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 19 Date: Tue, 22 Jun 2010 18:06:14 -0700 From: connie grubaugh <conniegruba...@hotmail.com> Subject: RE: [Histonet] Help! In need of positive Gram Control To: <cg...@marylandgeneral.org>, <jcbrit...@cheshire-med.com>, <dianar...@aol.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <snt104-w16f336b53be5a967c7507d6...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cg...@marylandgeneral.org > To: jcbrit...@cheshire-med.com; dianar...@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: dianar...@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > > dianar...@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5 ------------------------------ Message: 20 Date: Tue, 22 Jun 2010 21:50:42 EDT From: jmye...@aol.com Subject: RE: [Histonet] New CAP question ANP.22760 To: tjas...@copc.net Cc: histonet@lists.utsouthwestern.edu Message-ID: <7cdd9.6a7042fa.3952c...@aol.com> Content-Type: text/plain; charset="US-ASCII" Tom: As much as I agree with your acknowledgment that its seems a bit odd for the CAP to have a blood-banker responding to AP-related issue, I'm actually not surprised. The folks in the 'clinical' lab have been performing more comprehensive and complex validation procedures for a very long time, and they wonder why IHC isn't expected to follow the same requirements as chemistry, immunology, etc. -- IHC is, after all, an awful lot like ELISA. And rightfully so, because IHC is, under CLIA (which supersedes CAP), considered highly-complex, non-waived testing -- and is, therefore, subject to the same Quality Systems regulations (in particular, 42CFR493.1252-1256, 1273, and 1281) as the testing performed in other areas of the lab. Could it be that, because AP produces qualitative results that are interpreted by a pathologist and CP produces quantitative results that are interpreted by an analyzer, we somehow think that CLIA rules don't apply to IHC? I certainly don't have the answer to that, but it make me wonder what the future holds. As witnessed by some of the newest CAP 'standards' (including the question in question...no pun intended), e.g. ER/PR, where a minimum of 20 positive and 20 negative specimens must be tested, and where 10 of the positives must be weakly positive -- an acknowledgment that validation specimens must be carefully selected in order to obtain appropriate results), it certainly doesn't appear that the regulation of IHC testing is going to become more relaxed. Joe Myers, M.S., CT(ASCP) ------------------------------ Message: 12 Date: Fri, 18 Jun 2010 12:38:07 -0700 From: "Thomas Jasper" <tjas...@copc.net> Subject: RE: [Histonet] New CAP question ANP.22760 To: "Mark Tarango" <marktara...@gmail.com> Cc: _histo...@lists.utsouthwestern.edu_ (mailto:histonet@lists.utsouthwestern.edu) Mark, Did you notice the credentials from this CAP representative? MT with a Blood Bank specialty I believe. What I glean from that is...more than likely this person does not grasp the logistics of "contemporaneously" staining identical Abs from separate lots. She also likely does not understand the logistical application for detection and automation either. I'm not trying to be overly critical of this person. I'm sure she is quite intelligent and would not have the MT/SBB if she wasn't intelligent. It comes down to a lack of understanding Anatomic Pathology testing application re: automated IHC. I believe this is a common problem in and out of CAP. Many lab directors and other folks in positions of authority without AP/Histology/Cytology backgrounds seem to believe that broad clinical lab modalities apply to Anatomic Path scenarios. I used to refer to this in my former position as - "Trying to put the yoke of clinical lab onto anatomic path." We are laboratorians, but in many instances do not fit the general clinical lab mold. It's unfortunate that CAP has put this person in the position to respond. It is apparent to me that she's not grasping the particulars here. She probably never will unless she decides to go into a working, automated IHC "tissue" lab and take the time to ask questions and understand (learn) what we're all about. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, OR 97701 ------------------------------ Message: 21 Date: Wed, 23 Jun 2010 02:14:47 -0500 From: <susan.wal...@hcahealthcare.com> Subject: RE: [Histonet] Help! In need of positive Gram Control To: <conniegruba...@hotmail.com>, <cg...@marylandgeneral.org>, <jcbrit...@cheshire-med.com>, <dianar...@aol.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <4bf03f5404ebde409af9232da74b9ded2af9784...@fwdcwpmsgcms09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" Take some fresh tissue (we use umbilical cord) to micro and let them incubate in for a few days in gram + and/or negative. Then fix it and you will have good controls. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, June 22, 2010 9:06 PM To: cg...@marylandgeneral.org; jcbrit...@cheshire-med.com; dianar...@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! In need of positive Gram Control Tried the slim jim and all of my doctors did not like it. Don't waste your time. Connie G. > Date: Tue, 22 Jun 2010 10:16:14 -0400 > From: cg...@marylandgeneral.org > To: jcbrit...@cheshire-med.com; dianar...@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > CC: > > You have got to be kidding!! That's hysterical. So process a slim jim > and you have > Gram - and + controls. If you're serious I'm trying it. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie > Britton > Sent: Tuesday, June 22, 2010 6:10 AM > To: dianar...@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Help! In need of positive Gram Control > > > > > > Have you tried a Slim Jim? They have gram positive and negative rods in > > them. Regardless, I still enjoy eating them once and a while! > > > > > > > > Josie Britton Ht > > > > Cheshire Medical Center > > > > Keene, NH 03431 > > > > > > > > > > > > -----Original Message----- > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > > dianar...@aol.com > > Sent: Monday, June 21, 2010 7:43 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Help! In need of positive Gram Control > > > > > > > > Help! We are in need of positive Gram Control Blocks if anyone has any > > > > extra they are willing to part with. I have lots of Fungus, > > Pneumocystis and > > > > HPV tissue blocks to trade. > > > > > > > > Diana Ripley > > > > John Muir Histology > > > > Concord Campus > > > > 2540 East Street > > > > Concord, CA 94520 > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > CONFIDENTIALITY NOTICE: This electronic message, including any > attachments, is for the sole use of the intended recipients and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by electronic mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 23 Jun 2010 10:24:22 +0200 From: "Hoekert, W.E.J." <w.e.j.hoek...@olvg.nl> Subject: RE: [Histonet] Glut1 antibody To: "Martha Ward" <mw...@wfubmc.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <1190cb05c44b13409483514729c2fc360c0...@pait42.olvg.nl> Content-Type: text/plain; charset="iso-8859-1" We switched to Neomarkers (polyclonal) (RB 078-A). Willem Hoekert OLVG, Netherlands ________________________________ Van: histonet-boun...@lists.utsouthwestern.edu namens Martha Ward Verzonden: di 22-6-2010 22:10 Aan: histonet@lists.utsouthwestern.edu Onderwerp: [Histonet] Glut1 antibody I have been using anti-human Glut-1 from Dako for several years but when I went to order it I was told it was discontinued. Does anyone have a suggestion of where I can locate this antibody? Thanks in advance for your help! Martha Ward, MT (ASCP) QIHC Assistant Manager, Molecular Diagnostics Lab Wake Forest University Baptist Medical Center 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ------------------------------ Message: 23 Date: Wed, 23 Jun 2010 08:25:33 -0400 From: mtitf...@aol.com Subject: [Histonet] Retaining empty jars To: histo...@pathology.swmed.edu Message-ID: <8cce0e64f6a9c4a-fcc-6...@webmail-d119.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Rhonda Hartz asks if anyone else retains empty jars after grossing. We too retain our empty jars after grossing for about a month. We keep them in a red biohazard trash bag. It pays dividends. If once the case has been signed out the physician/surgeon complains that they removed more biopsies than we listed in the report, or submitted more jars, etc, we can go back and check. On rare occasions we have found tiny pieces of tissue in the jars, or hidden in the grooves of the lid. These enquiries most often happen with tiny pieces of tissue, of course, like GI biopsies. We have the physicians write on the label on the jar what is inside it (like everyone else I guess) as well as listing it on the surgical pathology slip. You can also go back and check what they wrote down. Afterwards the jars are incinerated as they have all that HIPPA related stuff on the labels. Michael Titford USA Pathology Mobile AL USA ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 79, Issue 28 **************************************** ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet