I read one paper or abstract recently where sections were made at 50
microns then used
successfully for RT-PCR. I am pretty sure that it was PRRS virus that
they were pursuing.
On 3:59, Mark Tarango wrote:
I would try getting more sections from LCM and extracting into 7.5 uL, if
that
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen organs. I fixed the
sections in EtOH70% for 10min and then I stained them with xGal for 3h at 37°C.
After air drying I cut out with the LCM and extract
Have you tried using more sections during extraction? Can you extract into
a smaller volume?
On Friday, November 2, 2012, Vanessa Orsini wrote:
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen
I would try getting more sections from LCM and extracting into 7.5 uL, if
that volume will be enough to for your PCR reactions.
On Friday, November 2, 2012, Vanessa Orsini wrote:
With the kit I'm extracting in 10ulthe problem is that I have too use
few stained cells isolated with the
: Friday, November 02, 2012 5:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RNA isolation form stained slides
Hello,
I need to extract RNA for a RT-PCR after Laser MicroDissection on xGal stained
slides.
I tried using sections from unfixed frozen organs. I fixed the sections
