Hi Miha. Sounds like pre-freezing artifact. Make sure that the isopentane and pre-cooled adequately and the specimens are frozen quickly.
On Mon, Oct 17, 2011 at 10:01 AM, <histonet-requ...@lists.utsouthwestern.edu > wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Looking for recommendations for a slide and cassette > labeller system (Linda) > 2. RE: (no subject) (Tony Henwood) > 3. RE: Hi histone (crochierest...@aol.com) > 4. processing schedule for GI'S (anuradha shrivastava) > 5. Re: pouring of liquid nitrogen into a cryostat (Tony Reilly) > 6. Technovit 9100 New (Megan Silas) > 7. Re: Technovit 9100 New (Jack Ratliff) > 8. RE: Recommendations for a slide and cassette labeler system > (D'Attilio, Shelley) > 9. HHV-6 Antibody (McMahon, Loralee A) > 10. RE: (no subject) (Bea DeBrosse-Serra) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 16 Oct 2011 11:12:55 -0700 (PDT) > From: Linda <lmd...@yahoo.com> > Subject: Re: [Histonet] Looking for recommendations for a slide and > cassette labeller system > To: Sheila Adey <sa...@hotmail.ca>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1318788775.94848.yahoomail...@web36506.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi, > > I use the Brady system, which has two dimension bar coding. They also have > a scan to print, which has a modem for wireless use. The customer service > is outstanding. The price is pretty reasonable too. > > > Linda Dee, BGS, HT(ASCP) > > > > ________________________________ > From: Sheila Adey <sa...@hotmail.ca> > To: histonet@lists.utsouthwestern.edu > Sent: Saturday, October 15, 2011 7:06 PM > Subject: [Histonet] Looking for recommendations for a slide and cassette > labeller system > > > Hello:We are looking into purchasing a slide and cassette labeller system. > Any advice would be great. Also, if you know which system to stay away from, > that would be appreciated aswell.ThanksSheila > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 2 > Date: Sun, 16 Oct 2011 22:34:09 +0000 > From: Tony Henwood <antho...@chw.edu.au> > Subject: RE: [Histonet] (no subject) > To: "'Miha Tesar'" <sauco...@yahoo.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <6d6bd1de8a5571489398b392a38a7157609f4...@xmdb02.nch.kids> > Content-Type: text/plain; charset="iso-8859-1" > > Miha, > > Are your muscles arising in saline? > Receive them dry or in cell culture media (eg Hanks). > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Laboratory Manager & Senior Scientist > Tel: 612 9845 3306 > Fax: 612 9845 3318 > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar > Sent: Saturday, 15 October 2011 7:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi! > I am working in Institute of Phatology Slovenia EU. Recently I have > problems with muscle biopsy. There are a lots of artefact that I can not > explain, like little holes in the tissue after the tissue is being frozen. > We are using the isopentan and the liquid nitrogen for the freezing. Can > enyone give me some ideas haw to avoid this artefact. How important is > humidity in laboratory for the good results? > The next problem is that after I put the tissue on the microscope > slides after coloring the GTK all the tissue gets wrinkeld. Iam using the > poly L lisin microscope slides or the Immuno microscope slides. > Thx in advance! > Best regards Miha from SLO > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************************************* > This email and any files transmitted with it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, please delete it and notify the > sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned and > although no computer viruses were detected, The Childrens Hospital at > Westmead accepts no liability for any consequential damage resulting from > email containing computer viruses. > > ********************************************************************************* > > > > ------------------------------ > > Message: 3 > Date: Sun, 16 Oct 2011 22:55:41 > From: crochierest...@aol.com > Subject: [Histonet] RE: Hi histone > To: histonet@lists.utsouthwestern.edu > Message-ID: 67750d312163f4fa9e5501866fb8429e@[192.168.1.1] > Content-Type: text/plain; charset="iso-8859-1" > > hey how have you been? Do you want to make some easy cash? > http://t.co/SEgZGeUm You've got to read this story! I will never have to > worry about the bills being paid on time again! > > > > ------------------------------ > > Message: 4 > Date: Sun, 16 Oct 2011 19:33:41 -0400 > From: anuradha shrivastava <shrivastavaanura...@hotmail.com> > Subject: [Histonet] processing schedule for GI'S > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <snt115-ds172b33a303245925541e07ad...@phx.gbl> > Content-Type: text/plain; charset="utf-8" > > Hello Histo gurus, > Could u please advice me on gi’s processing schedule for Leica asp 300. > thanks in advance. > > > ------------------------------ > > Message: 5 > Date: Mon, 17 Oct 2011 10:01:29 +1000 > From: "Tony Reilly" <tony_rei...@health.qld.gov.au> > Subject: Re: [Histonet] pouring of liquid nitrogen into a cryostat > To: "Louise Renton" <louise.ren...@gmail.com>, "Histonet" > <Histonet@lists.utsouthwestern.edu> > Message-ID: <4e9bfcf9.411c.003...@health.qld.gov.au> > Content-Type: text/plain; charset="us-ascii" > > Hi Louise > > Not a very safe practice. I have heard of people using a bag of dry ice > pellets rested against the knife holder to lower the temperature of the > knife, both for when the chamber is too warm or the tissue needs to be cut > at a lower temperature, such as fat. > > regards > Tony > > > > > Tony Reilly B.App.Sc. , M.Sc. > Chief Scientist, Anatomical Pathology > Pathology Queensland-PA Laboratory > _________________________________________________ > Clinical and Statewide Services Division| QueenslandHealth > > Level 1, Building 15,Princess Alexandra Hospital > Ipswich Road,WOOLLOONGABBA Qld4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > Fax: 07 3176 2930 > Email: tony_rei...@health.qld.gov.au > Web: www.health.qld.gov.au/qhcss/ > > > > > >>> Louise Renton <louise.ren...@gmail.com> 10/15/2011 2:59 am >>> > bad idea - what about splash ups from the chamber? Very scary to > contemplate > > On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan <kr...@nfderm.com> wrote: > > > Hi Everyone, > > > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > > cryostat that has warmed up to help cool it down more quickly? Does > > this do harm to the chamber or mechanisms of the cryostat? Any > > information would be appreciated. > > > > > > > > Thanks > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > +27 11 717 2298 (tel & fax) > 073 5574456 (emergencies only) > Question: Are rhinos overweight unicorns? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ******************************************************************************** > This email, including any attachments sent with it, is confidential and for > the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended recipient(s), > or if it is transmitted/received in error. > Any unauthorised use, alteration, disclosure, distribution or review of > this email is strictly prohibited. The information contained in this email, > including any attachment sent with it, may be subject to a statutory duty of > confidentiality if it relates to health service matters. > If you are not the intended recipient(s), or if you have received this > email in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer system network and > destroy any hard copies produced. > If not an intended recipient of this email, you must not copy, distribute > or take any action(s) that relies on it; any form of disclosure, > modification, distribution and/or publication of this email is also > prohibited. > Although Queensland Health takes all reasonable steps to ensure this email > does not contain malicious software, Queensland Health does not accept > responsibility for the consequences if any person's computer inadvertently > suffers any disruption to services, loss of information, harm or is infected > with a virus, other malicious computer programme or code that may occur as a > consequence of receiving this email. > Unless stated otherwise, this email represents only the views of the sender > and not the views of the Queensland Government. > > ********************************************************************************** > > > > ------------------------------ > > Message: 6 > Date: Sun, 16 Oct 2011 22:47:48 -0500 > From: Megan Silas <sil...@illinois.edu> > Subject: [Histonet] Technovit 9100 New > To: histonet@lists.utsouthwestern.edu > Message-ID: > <CADo6mFWahf4R9nMpQ+fjbKtV1HK8j1cz=l+bolszz0fzqqz...@mail.gmail.com > > > Content-Type: text/plain; charset=ISO-8859-1 > > Hello, > > Has anyone used Technovit 9100 New? If so, what company did you order > through - is there a distributor in the US? Also, have you found success > with this in embedding undecalcified bone? > > Thanks for any and all advice! > > Megan Silas > > Megan Silas | megan.r.si...@gmail.com | 847-609-6336 > University of Illinois at Urbana-Champaign | Bioengineering > > > ------------------------------ > > Message: 7 > Date: Sun, 16 Oct 2011 22:56:31 -0500 > From: Jack Ratliff <ratliffj...@hotmail.com> > Subject: Re: [Histonet] Technovit 9100 New > To: Megan Silas <sil...@illinois.edu> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <blu0-smtp43828af8cd45e5972e8a27dae...@phx.gbl> > Content-Type: text/plain; charset="us-ascii" > > What is it you are trying to accomplish with staining? Can you tell us more > about your project? I use Acrylosin SOFT Infiltration and Embedding > solutions from Dorn and Hart Microedge (just up the road from you in Villa > Park) for my thin 5 micron section and Acrylosin HARD for ground sections. > Feel free to ask more questions as needed. > > > Jack > > > Senior Histologist, BioMimetic Therapeutics > Chairman, Hard Tissue Committee - National Society for Histotechnology > > > > On Oct 16, 2011, at 10:47 PM, Megan Silas <sil...@illinois.edu> wrote: > > > Hello, > > > > Has anyone used Technovit 9100 New? If so, what company did you order > > through - is there a distributor in the US? Also, have you found success > > with this in embedding undecalcified bone? > > > > Thanks for any and all advice! > > > > Megan Silas > > > > Megan Silas | megan.r.si...@gmail.com | 847-609-6336 > > University of Illinois at Urbana-Champaign | Bioengineering > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 8 > Date: Mon, 17 Oct 2011 07:49:07 -0500 > From: "D'Attilio, Shelley" <sdatt...@stormontvail.org> > Subject: [Histonet] RE: Recommendations for a slide and cassette > labeler system > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > < > b7244ac045486c4190de73f226f432d4221fb...@exchange-vs1.stormontvail.org> > > Content-Type: text/plain; charset="iso-8859-1" > > Hi all, > Sheila asked for recommendations for a slide and cassette labeler system. > We use the Leica IPS and IPC printers and we are very happy with their > reliability and performance. I believe they are larger than other printers > but well worth the real estate, in my opinion. Our printers are interfaced > to our A/P LIS (WinSurge), but we also have the ability to print slides and > cassettes as needed through the printing software. Be advised that our > installation required that each printer be connected to its own PC and I > needed to purchase two interfaces from WinSurge, so it wasn't an inexpensive > project by any means. > > I think the Leica labelers are suitable for a high-volume laboratory, but > may be overkill for a lab with smaller volumes. > > Regards, > > Shelley D'Attilio MT(ASCP) > Manager, Chemistry, Cytology and Histology > Dept. of Pathology and Laboratory Medicine > Stormont-Vail HealthCare > Topeka, Kansas > > > NEED A DOCTOR? Stormont-Vail's Health Connections can help you find a > doctor accepting new patients. Call (785) 354-5225. > > > ****************************************************************************************************************** > > The information transmitted in this e-mail and in any replies and forwards > are for the sole use of the above individual(s) or entities and may contain > proprietary, privileged and/or highly confidential information. Any > unauthorized dissemination, review, distribution or copying of these > communications is strictly prohibited. If this e-mail has been transmitted > to you in error, please notify and return the original message to the sender > immediately at the above listed address. Thank you for your cooperation. > > > ****************************************************************************************************************** > > > > ------------------------------ > > Message: 9 > Date: Mon, 17 Oct 2011 09:17:33 -0400 > From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> > Subject: [Histonet] HHV-6 Antibody > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > < > ec3287d73321a14799bb96082de57b470db...@urmcms2.urmc-sh.rochester.edu> > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Histonet. > > Can anyone recommend a HHV-6 antibody to use in formalin fixed paraffin > embedded tissues. > Preferably ASR or IVD. But I'll take whatever suggestions. > Thanks in advance. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > > ------------------------------ > > Message: 10 > Date: Mon, 17 Oct 2011 07:12:45 -0700 > From: Bea DeBrosse-Serra <bdebrosse-se...@isisph.com> > Subject: RE: [Histonet] (no subject) > To: "'Miha Tesar'" <sauco...@yahoo.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <493caa64f203e14e8823737b9ee0e25f091e912...@exchmb01.isis.local> > Content-Type: text/plain; charset="iso-8859-1" > > Do you coat the muscle biopsies with talcum powder prior to freezing? > > Beatrice DeBrosse-Serra HT(ASCP)QIHC > Isis Pharmaceuticals > Antisense Drug Discovery > 1896 Rutherford Road > Carlsbad, CA 92008 > 760-603-2371 > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar > Sent: Saturday, October 15, 2011 1:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Hi! > I am working in Institute of Phatology Slovenia EU. Recently I have > problems with muscle biopsy. There are a lots of artefact that I can not > explain, like little holes in the tissue after the tissue is being frozen. > We are using the isopentan and the liquid nitrogen for the freezing. Can > enyone give me some ideas haw to avoid this artefact. How important is > humidity in laboratory for the good results? > The next problem is that after I put the tissue on the microscope > slides after coloring the GTK all the tissue gets wrinkeld. Iam using the > poly L lisin microscope slides or the Immuno microscope slides. > Thx in advance! > Best regards Miha from SLO > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 95, Issue 22 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet