See attached data sheet, we use pepsin enzyme digestion @ 37C for 30
minutes manually on FFPE
For Ventana Users Protease I for 16 minutes
Michael Ho, MLT
Resource Technologist(See attached file: CMV-E.pdf)
Immunopathology laboratory
Division of Pathology
DPLM
The Hospital for Sick Childr
Dear Jean,
I am very fond of Dako's M0854 and find I can use it at 1:400-1:500 when
pepsin is used as a pretreatment proteolytic enzyme as compared to HIER
pretreatment with a citrate buffer.
CMV is run everyday in my lab.
Jim Burchette
"Taylor, Jean"
Sent by: ih...@googlegroups.com
05/20/
