We do not use AE1/AE3 anymore. OSCAR single clone is a much better antibody. We retired AE1/AE3 out of fear that it will lead to false negative results in sentinel nodes.
Hadi On Oct 3, 2012, at 3:50 PM, Jackie Ferracone wrote: > Nice antibody, we use Proteinase-K, at a dilution of 1:500. Detection is with > DAKO’s ENV+ , and we use a DAKO autostainer. > > Jacqueline D. Ferracone > Research Associate, Pathobiology > University of Pennsylvania/New Bolton Center > Veterinary Medicine > 382 West Street Road > Kennett Square, PA, 19348-1692 > 610-925-6391 > 610-350-8860 (cell) > 610-925-6810 (fax) > > From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of > Taylor, Jean > Sent: Wednesday, October 03, 2012 3:47 PM > To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' > Subject: [IHCRG] CK AE1/AE3 > > Hi Everyone, > > I’d like some input as to what labs are using as a procedure for this > antibody. Is enzyme digestion or a form of antigen retrieval with heat used? > What dilution and detection system do you use as well as what type of machine > is it run on? > > Thanks for the info, > > Jean Taylor, HT (ASCP) QIHC > IHC Tech > Meriter Health Services > Madison, WI > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within > the National Society for Histotechnology. > > To post to this group, send email to ih...@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscr...@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: hi...@nsh.org or > call 443.535.4060. > > -- > You received this message because you are subscribed to the Google > Groups "ihcrg" group. The IHC Resource Group is a standing committee within > the National Society for Histotechnology. > > To post to this group, send email to ih...@googlegroups.com > To unsubscribe from this group, send email to > ihcrg+unsubscr...@googlegroups.com > For more options, visit this group at > http://groups.google.com/group/ihcrg?hl=en > > To contact the National Society for Histotechnology, email: hi...@nsh.org or > call 443.535.4060. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
