We use amylase digestion for our daily PAS/D stains. The procedure is not that complicated. We preheat the enzyme in a 45 degree C water bath for 10 minutes. Then we let the slides digest with the solution still in the water bath for 10 minutes. Then we rinse in running tap water for 5 minutes and proceed to the periodic acid. Nathan Jentsch BS HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
