Diana McCaig asked if anyone had a method for cresyl echt violet staining of Helicobacter.
This information was published in Histo-Logic in April 1999. This was all the information in the article. Cresyl Echt Violet (CEV) This method utilizes a metachromatic dye, cresyl echt violet (also called cresyl violet acetate) to stain the bacteria. The bacteria stand out well because the mucous layer does not stain heavily with the CEV and provides a nice contrast for the organism. It is a very easy and quick stain utilizing a 0.1% aqueous working solution of CEV. The slides are deparaffinized, hydrated to water, and stained in the CEV for 3 minutes. They are then rinsed in distilled water, dehydrated, cleared, and mounted. Reference: Gomes C. Rapid cresyl echt violet staining method for identifying Helicobacter pylori. On Stage, NY State Histotechnological Society. 1993;16(2). I recently had a locum tenens client who used this stain. I was not impressed that it was significantly better than the blue stain. They also had the immunostain available. I can probably get more information on the method from them. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet