Seconding Dr. Richmond's advice; a Wright-Giemsa stain is excellent for
highlighting bacteria.
Michael Baker, M.D.
CCHMC Pathology
Pager 736-1404
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Tanya G. Abbott RT (CSMLS), Manager Technologist, Histology/Cytology, St.
Joseph Medical Center, Reading PA asks:
We only perform a few Gram stains a year, hardly seems worth It to keep a
kit and expire reagents! Does anyone have an idea for other options? Ie,
doing the stains in Micro? Checking
Stacy Giroux (where?) asks: Our lab is currently transitioning from
the Brown Brenn gram stain due to no longer wanting to store picric
acid due to its potential hazards. Our pathologists have requested a
gram stain for paraffin embedded tissue that looks similar but does
not use picric acid or
@lists.utsouthwestern.edu
Subject: [Histonet] Re: Gram stain
Stacy Giroux (where?) asks: Our lab is currently transitioning from the Brown
Brenn gram stain due to no longer wanting to store picric acid due to its
potential hazards. Our pathologists have requested a gram stain for paraffin
embedded tissue
Stacy,
There are several Gram stain variants that do not use Picric acid.
Tworts variant uses fast green and neutral red after the crystal violet-Iodine
steps.
Brown-Hopps method uses Basic Fuchsin, Gallego's differentiator and 1.5%
Tartrazine after the crystal-violet steps, to stain the
The original Brown-Hopps tissue gram stain was published in Lee Luna's
AFIP Manual, 3rd ed. 1968. It did not require picric acid, ethyl
ether, or acetone. I remember that the stain was widely used after
that.
The AFIP manual method does require ethylene glycol monoethyl ether
(Cellosolve 1),
