Hi Jennifer,
We use low melt point agarose to pre-embed and it works consistently well with
our processing.
Material:
* 10% Neutral Buffered Formalin
* 1X Dulbeccos PBS
* NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080
: [Histonet] Re: Histogel
Histogel is indeed expensive, and often not permitted by Management
for that reason.
Preparing your own agar is a bit tricky - you really need a hot plate
and a magnetic stirrer, unlikely items in a histology lab. (2% or 3%
dry agar, in water.)
Back before hospital
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Milne,
Katy
Sent: Friday, June 24, 2011 12:27 PM
To: 'histonet@lists.utsouthwestern.edu'; 'mjdess...@wvhcs.org'
Subject: [Histonet] RE: HistoGel
We use histogel a lot in our lab. It's a research lab and we use it for
a few purposes
Histogel is indeed expensive, and often not permitted by Management
for that reason.
Preparing your own agar is a bit tricky - you really need a hot plate
and a magnetic stirrer, unlikely items in a histology lab. (2% or 3%
dry agar, in water.)
Back before hospital microbiology turned into the
We use histogel a lot in our lab. It's a research lab and we use it for a few
purposes - pelleting cultured cells then creating multi-culture TMAs for
testing antibodies and also pelleting cells from ascites and pleural effusions.
Has also been used to process really small samples that could
Currently doing Cryotomy on quickly frozen Golgi-Cox impregnated mouse
brains
at 100-120 microns. Cryoprotecting with 30% sucrose. Cutting at -15C.
Tried fresh tissue and brief NBF perfusion(5 min).
If I perfuse with NBF for 15 min, or immersion fix overnight, it seems to
destroy
spines and
