This message is for Casey Arnold, we never had any problems with our stainer/coverslipper, I will send you our procedure to try out. Do you dry slides on the stainer or elsewhere?
Andrea De Jager, H.T. ASCP Histology Manager, Regional Reference Lab Kaiser Permanente - Colorado Phone: 303-404-4152 Fax: 303-404-4161 email: andrea.x.deja...@kp.org NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Date: 06/13/2013 11:04 AM Subject: Histonet Digest, Vol 115, Issue 13 Sent by: histonet-boun...@lists.utsouthwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Tissue Processors (Matthew Roark) 2. Histology Openings (Anna Nolan) 3. Paraffin processing native sheep ACL (Elizabeth Ronan) 4. RE: Shandon Excelsior Tissue Processor (Tony Henwood (SCHN)) 5. RE: Shandon Excelsior Tissue Processor (Marcum, Pamela A) 6. Sakura Tissue-Tek Prisma (Casey Arnold) 7. RE: Control Storage (Bitting, Angela K.) 8. Old books (Amber McKenzie) 9. cutting arterial grafts (Megan Elizabeth Flynn) 10. RE: Electron Microscopy protocols (idimi...@mun.ca) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 Jun 2013 13:18:51 -0500 From: "Matthew Roark" <mro...@sfmc.net> Subject: [Histonet] Tissue Processors To: <histonet@lists.utsouthwestern.edu> Message-ID: <000f01ce6799$4a64f2b0$df2ed810$@net> Content-Type: text/plain; charset="us-ascii" So in the next couple of months we are getting ready to demo Thermo's STP 420ES and Leica's ASP6025 tissue processor . Looking for the good, bad, and ugly about them. So if you have one or have had a demo please share what you've found. Thanks!! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 <mailto:mro...@sfmc.net> mro...@sfmc.net <http://www.sfmc.net> http://www.sfmc.net ------------------------------ Message: 2 Date: Wed, 12 Jun 2013 15:19:34 -0400 From: "Anna Nolan" <ano...@prometheushealthcare.com> Subject: [Histonet] Histology Openings To: <histonet@lists.utsouthwestern.edu> Message-ID: <00c801ce67a1$c696cbd0$53c46370$@prometheushealthcare.com> Content-Type: text/plain; charset="us-ascii" Hi my name is Anna and I work with Prometheus Healthcare doing laboratory recruitment. Currently I'm looking to fill several histology positions. Please call or email me if any of these positions are of interest to you. Portland, ME -Histology Technologist (3AM-11AM) NYC -Grossing Specialist (3rd shift) -Histology Tech (1st shift) Suffern, NY -IHC Histology Tech (1st shift) -Cutting embedding, staining, and coverslipping. IHC Experience a plus. (7AM-4PM) Anna Nolan Recruiter Prometheus Healthcare Office 301-693-9057 Cell 301-693-8908 Fax 301-368-2478 <http://ano...@prometheushealthcare.com/> anolan <mailto:ch...@prometheushealthcare.com> @prometheushealthcare.com <http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6> http://www.linkedin.com/pub/annelise-nolan/55/ba0/ab6 <http://www.linkedin.com/profile/view?id=197035050> <http://www.prometheushealthcare.com/> www.prometheushealthcare.com ------------------------------ Message: 3 Date: Wed, 12 Jun 2013 16:59:52 -0400 From: Elizabeth Ronan <lizro...@umich.edu> Subject: [Histonet] Paraffin processing native sheep ACL To: histonet@lists.utsouthwestern.edu Message-ID: <51b8e148.3020...@umich.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, I need to paraffin process native sheep anterior cruciate ligament (ACL) that has been fixed in 10% neutral buffered formalin for 7 days. I was wondering if anyone with more expertise on this subject could guide me with the best lengths of times in the alcohols, xylenes, and paraffin to fix ACL. I tried a 12 hour program but the sections crumbled in the middle and it appeared that the paraffin had not fully perfused the ligament. I have access to the following program, and can alter the lengths of the steps for as long as desired: Program: 70% 80% 95% 95% 100% 100% Xylene Xylene Paraffin Paraffin Paraffin Any advice is much appreciated. Thanks for your time, Liz ------------------------------ Message: 4 Date: Wed, 12 Jun 2013 23:40:22 +0000 From: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au> Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "'Velez, Vanessa'" <vve...@usgs.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <6d6bd1de8a5571489398b392a38a71579d271...@xmdb04.nch.kids> Content-Type: text/plain; charset="us-ascii" Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* ------------------------------ Message: 5 Date: Thu, 13 Jun 2013 11:05:48 +0000 From: "Marcum, Pamela A" <pamar...@uams.edu> Subject: RE: [Histonet] Shandon Excelsior Tissue Processor To: "Tony Henwood (SCHN)" <tony.henw...@health.nsw.gov.au>, "'Velez, Vanessa'" <vve...@usgs.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <41d3a1af6fef0643bdc89e0516a6ea32ca170...@mail2node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Since we have some power issues here and 4 Excelsiors (no problems with them on this issue) we have UPS units on all of our computerized equipment. We did this due to issues in other areas of the lab and decided it was safer than taking a chance and being down. We bought Powervar and I think the suggested units were around $2,000 to $3,000 each with one unit serving two processors. Other sites in Little Rock who have some of the same power issues have had problems. Pam Marcum UAMS ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Tony Henwood (SCHN) [tony.henw...@health.nsw.gov.au] Sent: Wednesday, June 12, 2013 6:40 PM To: 'Velez, Vanessa'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Shandon Excelsior Tissue Processor Vanessa, We installed an Eaton 9130 UPS unit with a SSFIO Surge Protector. Cost under $1,500 Australian. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Velez, Vanessa Sent: Thursday, 13 June 2013 2:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shandon Excelsior Tissue Processor Does any one having problems with the Excelsior Tissue Processor power board repeatedly after power outage? This has happened to me three times in the last five years even though it has backup batteries. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Thu, 13 Jun 2013 08:15:23 -0500 From: Casey Arnold <carn...@ourlab.net> Subject: [Histonet] Sakura Tissue-Tek Prisma To: histonet@lists.utsouthwestern.edu Message-ID: <cafdw3oggzhvr7hrd32p27-mycwwusn-z7cxysk2z0ibarom...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We have recently started using a Sakura Tissue-tek Prisma that we inherited from our sister lab. While running some validation testing I have noticed that the staining is very inconsistent. The tissue will stain from light to dark within the same rack and sometimes within the same cut (slide). I have reset the mix, speed, etc, to factory settings. I have tried increasing my hematoxylin, and bluing times. I have changed water filters, decreased water rinse times, tried different types of tissue, all to no avail. Has anyone had this problem or have any suggestions on how to correct it? I have tried everything the Sakura tech line has recommended and am still at a loss. -- NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information. Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. Thank you *Casey Arnold * *Histology Supervisor** OURLabs / OPKO Diagnostics, LLC* 1450 Elm Hill Pike Nashville, TN 37210 Direct: 615-345-4582 Office: 615-874-0410 Fax: 615-345-4595 ------------------------------ Message: 7 Date: Thu, 13 Jun 2013 14:21:16 +0000 From: "Bitting, Angela K." <akbitt...@geisinger.edu> Subject: [Histonet] RE: Control Storage To: Elizabeth Chlipala <l...@premierlab.com>, "Herring, Colleen" <colleen_herr...@bshsi.org>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <77f52efab8b1694b885e277c48fcd0f647c72...@ghsexmbx4w8k1v.geisinger.edu> Content-Type: text/plain; charset="us-ascii" Something to remember is that humidity is the enemy too. If you are storing your slides in a refrigerator make sure that the humidity is well controlled. We found out the hard way. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 12, 2013 11:56 AM To: Herring, Colleen; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Control Storage We store our blocks at room temp, our IHC slides are stored in the fridge. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen [colleen_herr...@bshsi.org] Sent: Wednesday, June 12, 2013 9:48 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Control Storage Does anyone have any ideas or suggestions about the storage of blocks and precut slides for immunos? ________________________________________________________________________________________________________ The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. 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Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ________________________________________________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 8 Date: Thu, 13 Jun 2013 14:22:10 +0000 From: Amber McKenzie <amber.mcken...@gastrodocs.net> Subject: [Histonet] Old books To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5a33c952bb67f4468af1f36d739212bcbd6ad...@jerry.gia.com> Content-Type: text/plain; charset="us-ascii" I'm cleaning house :) Anyone interested in buying these old books? 1. A color Atlas of Histology by Dennis Strete, ISBN 0-06-501084-1 2. Human Histology, 2nd edition, by Alan Stevens and James Lowe, ISBN 0-7234-2485-3 3. Immunolgy, 4th edition, by Roitt, Brostoff, Male ISBN 0-7234-2178-1 4. Atlas of Histology with Funcitonal correlations, by Victor P. Erodschenko ISBN 0-583-2818-9 5. Histotechnology, A Self Instructional Text, 2nd edition, by Freida Carson (I'm interested in purchasing the 3rd edition). ------------------------------ Message: 9 Date: Thu, 13 Jun 2013 15:11:12 +0000 From: Megan Elizabeth Flynn <megan-fl...@northwestern.edu> Subject: [Histonet] cutting arterial grafts To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5748287483cd8a48bc4a27d05908d4e215e72...@chcspmbx3.ads.northwestern.edu> Content-Type: text/plain; charset="us-ascii" Hi all, I am in a research lab and I am cutting rat arterial grafts made with ePTFE. I'm cutting at 30 degrees at 5 microns. The lower edge of the graft seems to be lifting up from the adventitia and folding over. I have tried playing with the temperature, but none of it seems to make a difference. Can anyone help? Megan ------------------------------ Message: 10 Date: Thu, 13 Jun 2013 15:55:23 +0000 From: <idimi...@mun.ca> Subject: RE: [Histonet] Electron Microscopy protocols To: <joel.h...@gladstone.ucsf.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <14c3108e8b98ef43b3edae58336700341fa...@exchange.med.mun.ca> Content-Type: text/plain; charset="us-ascii" Hi Joel, Lipids are fixed only by OsO4, so you need to use it as a post-fixative. As a fixative in our lab we use Karnovsky fixative, then we use osmium tetroxide as a post-fixative/fixative for the lipids for 15 min. Then we wash in Na-cacodylate buffer for 5 min, and then dehydrate, starting with two changes of 70% ethanol ( for 10min.) and then we go to two changes of 95% and then absolute ethanol, followed by two changes of abs. acetone, then 50:50 acetone:resin and 2 changes of resin, then embedding and polymerization at the right temperature. If your lipids are fixed properly they will be round droplets with characteristic appearance. OsO4 will blacken the tissue. We always have excellent results with this protocol. Hope that was helpful, Iliana dimitrova, Memorial university of Newfoundland, Canada -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [ mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Haas Sent: June-05-13 3:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Electron Microscopy protocols Hello all, I am working on developing an electron microscopy protocol for looking at lipid droplets in cultured cells. We have used thin section TEM previously and found that the morphology of the droplets is often deformed (should be spherical, shows up as egg shaped or lumpy). If possible we would like to avoid these types of preservation artifacts. Does anyone have suggestions for adapting the protocol to better preserve these types of structures--a triglyceride lipid core surrounded by a phospholipid monolayer? Thanks in advance, Joel _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic communication is governed by the terms and conditions at http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2012.php ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 115, Issue 13 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet