On 3/25/10 1:04 PM, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: H & E QC (Sue) > 2. RE: H & E QC (WILLIAM DESALVO) > 3. Re: number of slides (Anne van Binsbergen) > 4. LOOKING FOR IHC POSITION (Isaac O) > 5. Re: H & E QC (Malika Benatti) > 6. RE: LOOKING FOR IHC POSITION (Plewinski, Amy) > 7. Symphony (Kelly Boyd) > 8. Job Opportunity (Jackie M O'Connor) > 9. A.O TP8000 Processor (Cheryl Crowder) > 10. RE: Symphony (CHRISTIE GOWAN) > 11. Re: Silver Lips and Fingers (Andrea Grantham) > 12. Troubleshooting IHC (Adam .) > 13. Benchmark Ultra (Troutman, Kenneth A) > 14. coverslipper (Margaryan, Naira) > 15. RE: coverslipper (Rathborne, Toni) > 16. Re: chick embryo frozen sections (Atoska Gentry) > 17. Re: Acetone Fixation (Atoska Gentry) > 18. RE: coverslipper (Nails, Felton) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 24 Mar 2010 23:02:35 +0000 (UTC) > From: Sue <suetp...@comcast.net> > Subject: Re: [Histonet] H & E QC > To: ADESUPO ADESUYI <adesupo2...@hotmail.com> > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <1021401464.930521269471755920.javamail.r...@sz0028a.westchester.pa.mail.comca > st.net> > > Content-Type: text/plain; charset=utf-8 > > We have automatic stainers, so after the stainer is set up a slide with a > micro-array is run. This slide is > checked by the histologists and logged in. The next slides run are our rapid > cases. A log sheet is > prepared and handed to the pathologist with the slides and they are graded for > processing, embedding, microtomy > and staining. This log is turned into the supervisor and reviewed daily. If > there are issues the supervisor will > review with the histologists. Since the techs rotate weekly we are able to > monitor all the tech's technical performance. > > Susan T. Paturzo > TJUH > > > > > ------------------------------ > > Message: 2 > Date: Wed, 24 Mar 2010 21:22:34 -0600 > From: WILLIAM DESALVO <wdesalvo....@hotmail.com> > Subject: RE: [Histonet] H & E QC > To: <adesupo2...@hotmail.com>, histonet > <histonet@lists.utsouthwestern.edu> > Message-ID: <blu103-w2204b7c6ba7d8c137df3f491...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > Whether you are using an automated stainer or hand staining, run a control > slide and review before any patient samples are stained. I also suggest that > only start or endpoint QC is not enough and you should consider incorporating > continuous QC/QA at regular intervals for the stain set-up, to ensure the > highest quality and provide adequate control of the process. You should be > able to determine, in a very short time, the end point of the stain set up and > then add QC checks for slide quality at 1/3 and 2/3 through the run or anytime > a solution container is changed or rotated. > > > > In our lab, with the regents used and staining protocols available to select, > we have determined that a stain set of solutions, on our automated instrument, > will maintain agreed and desired quality the pathologist will accept for 1500 > slides (I strongly suggest counting slides, not runs or racks). We stop > processing patient slides and run the control slide at runs 1, 500, 1000 (+- > 10% to allow for process flow and variance). The slides are reviewed for > acceptance or rejection by a Coordinator or higher and when acceptable,patient > slides may be placed on the instrument. All QC slides are saved for review and > the QC maintenance sheet is filed daily. This process captures the employee > that set up and monitors the instrument and the employee that QC'd along w/ > the QC review results. The control slide is a multi-tissue slide that must > contain the four highest volume tissue types for the lab. Each pathologist > receives a daily Quality Review sheet to report any variance, issues or > problems for all cases read. > > > > Think through your process, communicate with the pathologist and develop a > QC/QA system/process that creates accountability for all employees, supports > production of quality results and meets your regulatory needs. > > William DeSalvo, B.S., HTL(ASCP) > System Production Manager > > Sonora Quest Laboratories > > NSH Quality Control Committee Chairperson > > >> From: adesupo2...@hotmail.com >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 24 Mar 2010 17:53:12 -0400 >> Subject: [Histonet] H & E QC >>> >> Hi, >> >> I will appreciate it, if you guys could share your method/procedure for H & E >> QC with me. Thanking you all for your usual cooperation. >> >> >> >> Adesupo A. >> >> _________________________________________________________________ >> Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. >> http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL >> :en-US:WM_HMP:032010_1_______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. > http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAGL:ON:WL: > en-US:WM_HMP:032010_1 > > ------------------------------ > > Message: 3 > Date: Thu, 25 Mar 2010 13:34:12 +0400 > From: Anne van Binsbergen <anni...@gmail.com> > Subject: Re: [Histonet] number of slides > To: hymclab <hymclab.hymc...@ministryhealth.org> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <f8332fbe1003250234o100dadf4pe616d87dfbfc0...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Here is our basic microtomy protocol: > > BMT - 3 H&Es, each has a ribbon, plus PAS, Retic, Perls > Liver bx - 3 H&Es, each has a ribbon plus Retic, Trichrome, Perls, > Renal bx - 4 H&Es, each has a short ribbon plus PAS, PMS, trichrome - on > slides with gloms > Breast bx - 3 H&Es, each has a ribbon > Derm bx - 4 H&Es, each has a ribbon, one Path likes AP PAS on all punch bx's > GIT bx - 3 H&Es, each has a ribbon, plus HP > > all other small bx's get 3 H&Es, each with a ribbon > > and then we wait for the orders for levels and deepers and..and...and > > AbuDhabiAnnie > > > > On 25 March 2010 00:19, hymclab <hymclab.hymc...@ministryhealth.org> wrote: > >> We follow the same practice as Susan. Our Pathologists would rather have >> more than less!!! >> >> Dawn >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu [mailto: >> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue >> Sent: Tuesday, March 23, 2010 5:08 PM >> To: anita dudley >> Cc: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] number of slides >> >> We do three levels on all diagnostic biopsies. Liver and Kidney also get >> upfront special stains, as do gastric biopsies (h pylori) We did cut down on >> extra unstained slides since we were discarding most of them. As far as >> stopping levels, my pathologist thinks it goes against standard of care. As >> for prostate biopsies, they are so small any more, that we are thinking o >> cutting 4 slides staining 1and 4 for H&E and holding 2 and 3 for possible >> IHC. We are finding that when we have to go back there is minimal tumor for >> IHC demonstration. >> >> Susan T. Paturzo >> Thomas Jefferson University Hospital >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may >> contain confidential and privileged information for the use of the >> designated recipient(s) named above. If you are not the intended recipient, >> you are hereby notified that you have received this communication in error >> and that any review, disclosure, dissemination, distribution or copying of >> it or its contents is prohibited. If you have received this communication in >> error, please notify the sender at the electronic mail address noted above >> and destroy all copies of this communication and any attachments. Thank you >> for your cooperation. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet