Dear Colleagues, I would like to find chromogen for alkaline phosphatase enzyme labeling. I have used Vulcan fast red from Biocare, but it usually gives me background especially on double stained slides on mouse pancreas, i do KI 67 and BRDU/ Insulin double staining, and I use AP enzyme for the pancreatic islets with rabbit S Cruz anti insulin antibody and rabbit--on rodent AP polymer from Biocare Could you please share your experience what and from what company detection system and chromogen i can use to get rid of background. I appreciate any hints and protocols. Thank you. Galina Deyneko Novartis, Cambridge, MA 617-782-1675 home 617-871-7613 w
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