On all stomach biopsies. Tim ----- Original Message ----- From: <histonet-requ...@lists.utsouthwestern.edu> To: <histonet@lists.utsouthwestern.edu> Sent: Wednesday, July 13, 2011 10:45 PM Subject: Histonet Digest, Vol 92, Issue 18
Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Quick Drying Mounting Media (Katelin Lester) 2. Re: Quick Drying Mounting Media (Katelin Lester) 3. IHC for H. pylori (Joanne Clark) 4. Re: IHC for H. pylori (Patrick Laurie) 5. QC vs QA (H R) 6. RE: QC vs QA (Amber McKenzie) 7. Re: IHC for H. pylori (Richard Cartun) 8. Re: IHC for H. pylori (Mark Tarango) 9. RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX (Pam Barker) 10. Nbldsu (Paul Scholder) 11. And now, IHC playing cards! (mtitf...@aol.com) 12. Re: (Denise Piontek) 13. Question - Anatomic Pathology Manager (Richard Cartun) 14. Tzank Smears (Amy Self) 15. SlideMate Procedure (Laurie Colbert) 16. RE: IHC for H. pylori (Fawn Bomar) 17. Reference labs doing Fast Myosin IHC stain (Sebree Linda A) 18. Re: QC vs QA (SHANE NELSON) 19. Bone Marrow Biopsies (Heather Cooper) 20. out of office (marilyn.a.we...@kp.org) 21. Antigen retrieval survey (Andrea T. Hooper) 22. Re: confocal questions: mixing fluorescent dyes with proteins (Andrea T. Hooper) 23. Re: In search of a cryostat... (Andrea T. Hooper) 24. Anti-RFP (Andrea T. Hooper) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 Jul 2011 10:42:25 -0700 From: Katelin Lester <katelin09...@gmail.com> Subject: [Histonet] Quick Drying Mounting Media To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <CAOEzYbS9j+_CT5UWZ5Hh9CiXT8wEXCyqLDuiNCrYij2=wat...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi histonet, We have a brand new Leica CV5030 Coverslipper and I am looking for the quickest drying media available. We are currently using Micromount by Surgipath/Leica and it is not drying fast enough. Any suggestions are appreciated as we have to package our slides for shipping and the media is either sticking to the bubble wrap or creating circles on the slides from the pressure of the bubble wrap. -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ------------------------------ Message: 2 Date: Wed, 13 Jul 2011 11:14:01 -0700 From: Katelin Lester <katelin09...@gmail.com> Subject: Re: [Histonet] Quick Drying Mounting Media To: Marcia Funk <fu...@mercyhealth.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <caoezybtbrtaisapoc9mvl7mz7ruawq2ohqmcbszmvoeytoo...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We are shipping them out same day. At this point, if I can not find a quicker drying media I will have to delay the shipments by one day. What temperature do you dry them at? On Wed, Jul 13, 2011 at 10:50 AM, Marcia Funk <fu...@mercyhealth.com> wrote: > Katelin- do you ship the dame day ? We dry our slides in a oven daily > and file > ship the next day . seems to work > Marcia > > Marcia Funk > Histology Laboratory > Mercy Medical Center North Iowa > Mason City, IA, 50401 > 641-428-7907 > > > >>> Katelin Lester <katelin09...@gmail.com> 7/13/2011 12:42 PM >>> > > Hi histonet, > We have a brand new Leica CV5030 Coverslipper and I am looking for the > quickest drying media available. We are currently using Micromount by > Surgipath/Leica and it is not drying fast enough. > Any suggestions are appreciated as we have to package our slides for > shipping and the media is either sticking to the bubble wrap or creating > circles on the slides from the pressure of the bubble wrap. > > > -- > Katelin Lester, HTL > Gastroenterology Specialists of Oregon, P.C. > Pathology Laboratory > (971) 224-2408 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Katelin Lester, HTL Gastroenterology Specialists of Oregon, P.C. Pathology Laboratory (971) 224-2408 ------------------------------ Message: 3 Date: Wed, 13 Jul 2011 18:22:17 +0000 From: Joanne Clark <jcl...@pcnm.com> Subject: [Histonet] IHC for H. pylori To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <0494a7d4e8cc254ea2fb81464982e3786e6...@s10maild001n1.sh10.lan> Content-Type: text/plain; charset="us-ascii" Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico ------------------------------ Message: 4 Date: Wed, 13 Jul 2011 11:29:24 -0700 From: Patrick Laurie <foreig...@gmail.com> Subject: Re: [Histonet] IHC for H. pylori To: Joanne Clark <jcl...@pcnm.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <CAKEyg-3G93rn1fSVEJCtY9TZsuAECFkoQhc=nbzb-ls0fp0...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark <jcl...@pcnm.com> wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com ------------------------------ Message: 5 Date: Wed, 13 Jul 2011 14:29:30 -0400 From: H R <hrfulk...@gmail.com> Subject: [Histonet] QC vs QA To: histonet@lists.utsouthwestern.edu Message-ID: <CAF1LQBCGaWdTEtvtCiMdmsYxNLmz=2yamtxkhrihxetpcks...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? ------------------------------ Message: 6 Date: Wed, 13 Jul 2011 13:32:08 -0500 From: "Amber McKenzie" <amber.mcken...@gastrodocs.net> Subject: RE: [Histonet] QC vs QA To: <histonet@lists.utsouthwestern.edu> Message-ID: <03c921a1eaf7f541b16543f6ec6a4b370448a...@giamail2.gia.com> Content-Type: text/plain; charset="us-ascii" If you only need to keep QC records for 2 years, is it the same for QA records? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of H R Sent: Wednesday, July 13, 2011 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 13 Jul 2011 14:37:50 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: Re: [Histonet] IHC for H. pylori To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Joanne Clark" <jcl...@pcnm.com> Message-ID: <4e1dadbe.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII We only run IHC for H. pylori when the appropriate inflammatory background is present or when the patient has tested positive in the past and we receive a follow-up gastric specimen where bugs are not identified on H&E. Running H. pylori IHC on every gastric biopsy is uncalled for and borders on "Fraud and Abuse". Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> Joanne Clark <jcl...@pcnm.com> 7/13/2011 2:22 PM >>> Hi All, was wondering how everyone does their IHC for H. pylori. Do you automatically run it on all biopsies of the stomach, or do you screen first with a giemsa and run IHC on the ones that are negative by giemsa? Thanks for the input! Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 13 Jul 2011 11:47:13 -0700 From: Mark Tarango <marktara...@gmail.com> Subject: Re: [Histonet] IHC for H. pylori To: Richard Cartun <rcar...@harthosp.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Joanne Clark <jcl...@pcnm.com> Message-ID: <cacjwj1+qqsw9meiuzkcns720a59lqcz7wkazsdaicffzyi1...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We run the H. pylori IHC stain on all stomach specimens to improve turn around time; however, when an inflammatory background is not appreciated by the pathologist the charge is removed before sign-out and we eat the cost of producing the slide. Mark On Wed, Jul 13, 2011 at 11:37 AM, Richard Cartun <rcar...@harthosp.org>wrote: > We only run IHC for H. pylori when the appropriate inflammatory background > is present or when the patient has tested positive in the past and we > receive a follow-up gastric specimen where bugs are not identified on H&E. > > Running H. pylori IHC on every gastric biopsy is uncalled for and borders > on "Fraud and Abuse". > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > >>> Joanne Clark <jcl...@pcnm.com> 7/13/2011 2:22 PM >>> > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Wed, 13 Jul 2011 15:11:29 -0400 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Hot Management Job Alert Anatomic Pathology Lab Manager for top Lab in Austin, TX To: "'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <756593A6AD9F49EFA099D740BFD653AB@ownerf1abaad51> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters!! Here is a an exciting opportunity HOT OFF THE PRESSES! Anatomic Laboratory Manager - Leading Diagnostic Lab - Austin, TX RELIA Solutions the nation's only recruiting firm specializing in the permanent placement of histology professionals has been engaged by a leading Pathology Lab in Austin, TX to assist in their search for an Anatomic Pathology Laboratory Manager. My client offers a very competitive compensation package including bonuses and relocation assistance along with an incredible benefits package. This person will be responsible for the overall daily and financial operation of the Histology and Cytology Departments. Direct supervision of their department supervisors, delegating responsibilities as appropriate to these General Supervisors. Working directly in support of the Technical supervisors for these areas. As well as developing or approving department specific policies. Participating in the development and approval of laboratory wide policies and procedures; ensuring that all laboratory policies and procedures are followed; accepting and supporting activities delegated by the Medical Director and Vice President of Operations. Qualifications: 1.Bachelors or equivalent degree in the sciences. 2.Minimum 10 years experience in laboratory operations. 3.ASCP certification or equivalent for testing areas. 4.Leadership skills and supervisory abilities. 5.Organize and coordinate diverse activities. 6.Continuous improvement experience For more information contact Pam Barker at rel...@earthlink.net or toll free at 866-607-3542. Keywords histology histologist anatomic pathology cytology Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 10 Date: Wed, 13 Jul 2011 14:14:18 -0500 From: Paul Scholder <pa...@ncimicro.com> Subject: [Histonet] Nbldsu To: Histonet@lists.utsouthwestern.edu Message-ID: <ca+m6khkews8rvf8ib+e9-adm-wryabvtwz3a4a2rfuz-ekz...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 ------------------------------ Message: 11 Date: Wed, 13 Jul 2011 15:18:55 -0400 From: mtitf...@aol.com Subject: [Histonet] And now, IHC playing cards! To: histonet@lists.utsouthwestern.edu Message-ID: <8ce0fa8d804645b-2794-3...@webmail-d180.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Those of you that like freebies (like Dako mousepads, Thermo pens and Sigma coffee mugs) will be amused to learn that Cell Marque are giving away packs of playing cards. Each card shows a section stained with a different antobody (one of theirs of course, together with the catalogue number). No, I don't have shares in Cell Marque, but I am continually amused by the different methods companies in all walks of life use to advertise their products. Some are, well, just original! Michael Titford Pathology USA Mobile AL USA ------------------------------ Message: 12 Date: Wed, 13 Jul 2011 15:23:32 -0400 From: Denise Piontek <dbpion...@hotmail.com> Subject: [Histonet] Re: To: <camille_di...@bio-rad.com>, <cdi...@gmail.com>, <canvaspeo...@email.canvaspeople.com>, <dpant...@hrecruiters.com>, <deedra...@yahoo.com>, <histonet@lists.utsouthwestern.edu>, <i...@littlepeopleplayhouse.com> Message-ID: <col112-w1130c298cb68d864d1fbc7ab...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" It's the most powerful treatment I've ever used!... http://israelnlp.com/inbox.php?epyahooID=76n4 ------------------------------ Message: 13 Date: Wed, 13 Jul 2011 15:27:55 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: [Histonet] Question - Anatomic Pathology Manager To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <4e1db97b.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII How many Hospital-based Pathology Departments with 55,000 surgical specimens, 100,000 PAPs, and 6,000 non-GYN cytology cases have separate managers for Cytology and Histology? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ------------------------------ Message: 14 Date: Wed, 13 Jul 2011 15:42:38 -0400 From: Amy Self <as...@georgetownhospitalsystem.org> Subject: [Histonet] Tzank Smears To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <eeed2ff216617d45aff64bf53ec4f9c712be04a...@gmhdtcexch.gmhpost.com> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, I was wandering if anyone had a SOP for the collection and processing/staining of tzank smears that they would be willing to share with me? Thanks in advance for everyone's help. Amy Self Georgetown Hospital System Dept. of Pathology NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 15 Date: Wed, 13 Jul 2011 12:46:31 -0700 From: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com> Subject: [Histonet] SlideMate Procedure To: <Histonet@lists.utsouthwestern.edu> Message-ID: <57be698966d5c54eae8612e8941d76830ad2a...@exchange3.huntingtonhospital.com> Content-Type: text/plain; charset="us-ascii" Does anyone have a PSLIM/SlideMate procedure that they would be willing to share with me? Laurie Colbert ------------------------------ Message: 16 Date: Wed, 13 Jul 2011 16:07:01 -0400 From: Fawn Bomar <fawn.bo...@halifaxregional.com> Subject: RE: [Histonet] IHC for H. pylori To: Patrick Laurie <foreig...@gmail.com>, Joanne Clark <jcl...@pcnm.com> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <e779667a9118f74d8b7d82a1891be030e44797e...@exch-2k7.hrhs.com> Content-Type: text/plain; charset="us-ascii" We run giemsa's on all stomach bx's ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie [foreig...@gmail.com] Sent: Wednesday, July 13, 2011 2:29 PM To: Joanne Clark Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC for H. pylori *** WARNING *** This email has already passed extensive Anti-Spam and Anti-Virus scanning measures and found to have a high probability of not containing spam or virus content. However... This email has failed Domain Keys Identified Mail (DKIM) verification. DKIM references can be found at http://www.dkim.org and is defined by the overseeing organization as: "DomainKeys Identified Mail (DKIM) lets an organization take responsibility for a message while it is in transit. The organization is a handler of the message, either as its originator or as an intermediary. Their reputation is the basis for evaluating whether to trust the message for delivery. Technically DKIM provides a method for validating a domain name identity that is associated with a message through cryptographic authentication. " In this email, a DKIM key was supplied in the email and the DKIM cryptographic authentication has failed (with 100% certainty). Due to this, you should not necessarily trust that it was really sent from the domain stated by the sender "Patrick Laurie <foreig...@gmail.com>/histonet-boun...@lists.utsouthwestern.edu". 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If you need further information concerning this classification, please contact CareBridge Support with the below information: Filter: ib_dkim_fail Policy: $THROTTLED-conn_accept Time: 14:30:17 Message ID: 56429152 Envelope From: histonet-boun...@lists.utsouthwestern.edu Envelope Recipients: donna.hun...@northside.com, terri.br...@northside.com, fawn.bo...@halifaxregional.com >From IP: swlx162.swmed.edu --- Headers --- From histonet-boun...@lists.utsouthwestern.edu Wed Jul 13 14:30:17 2011 X-IronPort-RCPT-TO: donna.hun...@northside.com X-IronPort-RCPT-TO: terri.br...@northside.com X-IronPort-RCPT-TO: fawn.bo...@halifaxregional.com Received: from swlx162.swmed.edu ([199.165.152.162]) by ironport2.carebridge.net with ESMTP; 13 Jul 2011 14:30:16 -0400 Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Bh-0004Ss-Ai; Wed, 13 Jul 2011 13:29:33 -0500 Received: from [199.242.239.114] (helo=swlxmx2.swmed.edu) by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Qh4Be-0004Sn-Bw for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: from mail-gy0-f170.google.com ([209.85.160.170]) by swlxmx2.swmed.edu with esmtps (TLSv1:RC4-SHA:128) (Exim 4.76) (envelope-from <foreig...@gmail.com>) id 1Qh4BZ-0003KS-0v for histonet@lists.utsouthwestern.edu; Wed, 13 Jul 2011 13:29:30 -0500 Received: by gyb13 with SMTP id 13so4681932gyb.1 for <histonet@lists.utsouthwestern.edu>; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=gmail.com; s=gamma; h=mime-version:in-reply-to:references:date:message-id:subject:from:to :cc:content-type; bh=lOG2hfqGVG6xcFwEvpzyuF7pMx4zd9N+bkiO1fp2Hu4=; b=lMlgbSyOHSzOkPu4h9jvfu3Tthsx82aI3mQrNy2pGaByT6kLNNTbnKGb4YTCMT9JN/ OtZItQexDVs9Vj2s0qj0TsYSMvL5Te3b4nvutmrrdzV5obG20Cs40cn0Pvx6xEb3QhXO 80KaRKs+mQ4pE8cp6U38LCIZYowV53YdafTK4= MIME-Version: 1.0 Received: by 10.101.214.10 with SMTP id r10mr1372056anq.115.1310581764172; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) Received: by 10.100.110.18 with HTTP; Wed, 13 Jul 2011 11:29:24 -0700 (PDT) In-Reply-To: <0494a7d4e8cc254ea2fb81464982e3786e6...@s10maild001n1.sh10.lan> References: <0494a7d4e8cc254ea2fb81464982e3786e6...@s10maild001n1.sh10.lan> Date: Wed, 13 Jul 2011 11:29:24 -0700 Message-ID: <CAKEyg-3G93rn1fSVEJCtY9TZsuAECFkoQhc=nbzb-ls0fp0...@mail.gmail.com> From: Patrick Laurie <foreig...@gmail.com> To: Joanne Clark <jcl...@pcnm.com> X-Scan-Signature: e86858519762891a1be11808e8cb03b9 X-Spam-Checker-Version: SpamAssassin 3.3.1 (2010-03-16) on swlxmx2.swmed.edu X-Spam-Level: X-Spam-Status: No, score=-0.6 required=5.0 tests=DKIM_SIGNED,DKIM_VALID, DKIM_VALID_AU, FREEMAIL_FROM, HTML_MESSAGE, J_CHICKENPOX_16, RCVD_IN_DNSWL_LOW, SPF_PASS autolearn=disabled version=3.3.1 X-Spam-Relay-Country: US Subject: Re: [Histonet] IHC for H. pylori Content-Type: text/plain; charset=ISO-8859-1 X-Content-Filtered-By: Mailman/MimeDel 2.1.5 Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> X-BeenThere: histonet@lists.utsouthwestern.edu X-Mailman-Version: 2.1.5 Precedence: list List-Id: For the exchange of information pertaining to histotechnology and related fields <histonet.lists.utsouthwestern.edu> List-Unsubscribe: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>, <mailto:histonet-requ...@lists.utsouthwestern.edu?subject=unsubscribe> List-Archive: <http://lists.utsouthwestern.edu/pipermail/histonet> List-Post: <mailto:histonet@lists.utsouthwestern.edu> List-Help: <mailto:histonet-requ...@lists.utsouthwestern.edu?subject=help> List-Subscribe: <http://lists.utsouthwestern.edu/mailman/listinfo/histonet>, <mailto:histonet-requ...@lists.utsouthwestern.edu?subject=subscribe> Sender: histonet-boun...@lists.utsouthwestern.edu Errors-To: histonet-boun...@lists.utsouthwestern.edu X-Scan-Signature: f1c10eec54d1b063b9d41be68e9394bc X-SA-Exim-Connect-IP: 127.0.0.1 X-SA-Exim-Mail-From: histonet-boun...@lists.utsouthwestern.edu X-SA-Exim-Scanned: No (on swlx162.swmed.edu); SAEximRunCond expanded to false ************* Received Email Follows ************* Our pathology groups decision was to run IHC for H.pylori on all stomach biopsies. On Wed, Jul 13, 2011 at 11:22 AM, Joanne Clark <jcl...@pcnm.com> wrote: > Hi All, was wondering how everyone does their IHC for H. pylori. Do you > automatically run it on all biopsies of the stomach, or do you screen first > with a giemsa and run IHC on the ones that are negative by giemsa? Thanks > for the input! > > Joanne Clark, HT > Histology Supervisor > Pathology Consultants of New Mexico > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Patrick Laurie HT(ASCP)QIHC CellNetix Pathology & Laboratories 1124 Columbia Street, Suite 200 Seattle, WA 98104 plau...@cellnetix.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------- This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ------------------------------ Message: 17 Date: Wed, 13 Jul 2011 15:27:48 -0500 From: "Sebree Linda A" <lseb...@uwhealth.org> Subject: [Histonet] Reference labs doing Fast Myosin IHC stain To: <Histonet@lists.utsouthwestern.edu> Message-ID: <f0b9fd4b28c80e43996ffd4408b198aea5b...@uwhc-mail3.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="us-ascii" I'm looking for a reference lab that can do Fast Myosin immunohistochemistry staining on FFPE human tissue. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 ------------------------------ Message: 18 Date: Wed, 13 Jul 2011 13:38:17 -0700 (PDT) From: SHANE NELSON <nelsonr...@verizon.net> Subject: Re: [Histonet] QC vs QA To: Histonet <Histonet@lists.utsouthwestern.edu> Message-ID: <1310589497.49973.yahoomai...@web84308.mail.re1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Quality Assurance outlines all required QC activities used to identify policies or procedures that are not working as desired, especially those that are not immediately apparent from following routine QC procedures. The purspose of a QA plan is effectively achieved by providing a mechanism for identifying problems so that corrective actions can be taken to ensure, reliable, and prompt reporting of patient test results. Quality Control is to assess the effectiveness of the quality control system to identify inaccuracies in test systems prior to result reporting. To verify the proper use and evaluation of quality control data by testing personnel. To assess test methodologies based upon past QC results. THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net ________________________________ From: H R <hrfulk...@gmail.com> To: histonet@lists.utsouthwestern.edu Sent: Wed, July 13, 2011 11:29:30 AM Subject: [Histonet] QC vs QA Working on my procedure manual for CLIA and was wondering what the difference is between QC procedures and QA procedures.? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Wed, 13 Jul 2011 13:41:46 -0700 (PDT) From: Heather Cooper <hctrup...@att.net> Subject: [Histonet] Bone Marrow Biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <1310589706.46384.yahoomai...@web80013.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii What is the procedure for collecting and processing bone marrow bx? How much time should it be placed in DECAL?????????????? ------------------------------ Message: 20 Date: Wed, 13 Jul 2011 16:02:55 -0700 From: marilyn.a.we...@kp.org Subject: [Histonet] out of office To: histonet@lists.utsouthwestern.edu Message-ID: <of2b401e0e.d37a61ef-on882578cc.007e9c49-882578cc.007e9...@kp.org> Content-Type: text/plain; charset=US-ASCII I will be out of the office starting 07/13/2011 and will not return until 07/18/2011. In my absence please ask for Mary . If this is urgent or you need to speak to me directly you can contact me on my cell phone number 858-472-4266. If it concerns a Mohs to be scheduled you can e-mail me or call on my cell. Thank you. ------------------------------ Message: 21 Date: Wed, 13 Jul 2011 20:10:16 -0700 (PDT) From: "Andrea T. Hooper" <andreahoo...@rocketmail.com> Subject: [Histonet] Antigen retrieval survey To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1310613016.98546.yahoomailclas...@web113107.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi All, I am doing a survey and will be happy to compile results and share if folks will respond! What is your favorite antigen retrieval method and/or panel? Buffer Source/composition Temperature Device Thanks, Andrea ------------------------------ Message: 22 Date: Wed, 13 Jul 2011 20:13:43 -0700 (PDT) From: "Andrea T. Hooper" <andreahoo...@rocketmail.com> Subject: Re: [Histonet] confocal questions: mixing fluorescent dyes with proteins To: tgen...@gmail.com Cc: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1310613223.80664.yahoomailclas...@web113107.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 It will depend on how your scope is configured. On our system, when using the three you have outlined I routinely use CY5 or Alexa633 (or equivalent in far red category) on our scope. It's actually my personal favorite. If you have any doubts check out the spectral viewer on Invitrogen and also BD Bioscience's website. --- On Wed, 7/13/11, Tyrone Genade <tgen...@gmail.com> wrote: From: Tyrone Genade <tgen...@gmail.com> Subject: [Histonet] confocal questions: mixing fluorescent dyes with proteins To: "histonet" <histonet@lists.utsouthwestern.edu> Date: Wednesday, July 13, 2011, 11:23 AM Hello, I'm working on a Zeiss confocal microscope. We use chiefly Alexa488 and Cy3 together with Hoechst 33258. Can anyone suggest a fluorescent protein which can be discerned amongst the Alexa488, Cy3 and Hoechst 33258 emission spectra? I was thinking maybe mBanana? Thanks -- Tyrone Genade email: tgen...@gmail.com tel: +27-84-632-1925 (c) Department of Human Biology University of Cape Town South Africa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 23 Date: Wed, 13 Jul 2011 20:17:03 -0700 (PDT) From: "Andrea T. Hooper" <andreahoo...@rocketmail.com> Subject: Re: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, Tom McNemar <tmcne...@lmhealth.org>, Rene J Buesa <rjbu...@yahoo.com> Message-ID: <1310613423.83362.yahoomailclas...@web113106.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Agree, switching to Leica with disposable blade holder changed my life. Never looked back. Love the CM3050S. --- On Sun, 7/10/11, Rene J Buesa <rjbu...@yahoo.com> wrote: From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, "Tom McNemar" <tmcne...@lmhealth.org> Date: Sunday, July 10, 2011, 3:55 PM Buy Leica René J. --- On Sun, 7/10/11, Tom McNemar <tmcne...@lmhealth.org> wrote: From: Tom McNemar <tmcne...@lmhealth.org> Subject: [Histonet] In search of a cryostat... To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Date: Sunday, July 10, 2011, 10:53 AM Hello all, I am looking to budget for a new cryostat and was wondering what others are using. I would like one that is safe, well lit, roomy, and has some sort of on-board decontamination routine. All input appreciated. Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org<mailto:tmcne...@lmhealth.org> www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Application% 20Data\Microsoft\Signatures\www.LMHealth.org> ________________________________ This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Wed, 13 Jul 2011 20:40:16 -0700 (PDT) From: "Andrea T. Hooper" <andreahoo...@rocketmail.com> Subject: [Histonet] Anti-RFP To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1310614816.57254.yahoomailclas...@web113116.mail.gq1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Is anyone currently staining for RFP in FFPE or frozen sections using a detecting antibody? I am curious as to what anti-RFP Ab would be recommended. There is one I plan to try from Invitrogen (rabbit anti-RFP) but of course would love feedback if people have used one in particular. Thanks, Andrea ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 92, Issue 18 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet