Michele, This is our procedure for toe & finger nails; 1) Pre-fix nails in 10% NFB as usual 2) Soak fixed nails in NAIR or any counter nail remover until soften or bend easily (toe nail take longer; thickness dependent) 3) wash nail with water 4) Process in tissue processor 5) embed & cut Note; we found soften with NAIR before processing work the best.
If NAIR is used after processing; 1) cut nail on charge slides (+) 2) put slides in a plastic coplin jar with ~ 1cc 10% NFB & close cover tightly 3) bake jar in ~ 60c over for ~ 30 min 4) cool & open jar in fume hood ~ 5 min 5) stain as usual Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org On Sat, Jan 7, 2012 at 10:00 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. finger nails (Michele Carr) > 2. Temporary & Direct hire PA(ASCP) for February/March (Refer a > Friend) (Cheryl) > 3. Saffron (Gagnon, Eric) > 4. Re: finger nails (Rene J Buesa) > 5. Quality assurance program for pathologists (Diana McCaig) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 6 Jan 2012 10:06:40 -0800 (PST) > From: Michele Carr <michelecar...@yahoo.com> > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <1325873200.90291.yahoomail...@web120704.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi everyone was wondering what you do to get the nail from washing off the > slide during staining. The nail is extremely hard and seems to be washing > each time. Could I soften it prior to staining and what do you use to soften > it? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > > ------------------------------ > > Message: 2 > Date: Fri, 6 Jan 2012 10:51:12 -0800 (PST) > From: Cheryl <tkngfl...@yahoo.com> > Subject: [Histonet] Temporary & Direct hire PA(ASCP) for > February/March (Refer a Friend) > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1325875872.67239.yahoomail...@web39404.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello 'Netters- > > Do you know any temp/travel PAs? We've got a couple of different openings > for the right people. In this economy who doesn't want a few more > options??!! If you refer someone we help, you get a referral bonus (keep it > or share it with your friend!) > > 1. Temp traveler for at least 4 weeks in mid-February. > 2. Direct Hire in 6 different institutions around the US. Entry through > Supervisory. > > As a working tech I know a lot about the jobs before we submit you--and we > respect that it's your life we're talking about--we help make sure you have > lots of choices: you decide. > > Give me a call--make sure those you refer mention your name--I LIKE writing > referral checks! > > Cheryl > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one GREAT Tech at a time. > 281.852.9457 Office > 800.756.3309 Phone & Fax > ad...@fullstaff.org > > Sign up for the FREE newsletter AP News--updates, tricks of the trade and > current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' > request to apn...@fullstaff.org. Please include your name and specialty in > the body of the email. > > ------------------------------ > > Message: 3 > Date: Fri, 6 Jan 2012 15:19:18 -0500 > From: "Gagnon, Eric" <gagn...@kgh.kari.net> > Subject: [Histonet] Saffron > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <f93bd6329fc3ae4c8db116b985fbc3134d1d4...@kghmail.kgh.on.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Beth, as Bob Richmond has noted regarding saffron, > > "The most common use is as the hematoxylin-phloxin-saffron (HPS) > trichrome stain. It was in use as a general oversight stain in a few > pathology services when I was a resident in the 1960's..." > > and is still in use here in Ontario by about 10% of the province's histology > laboratories as a routine oversight stain. > > We have gone the same route as other respondents have noted over the years, > utilizing a variety of suppliers, including a Mediterranean health food store > in Ottawa for saffron. Now we are using the Sun Brand saffron produced in > Spain, that is available at the check-out counter at our local bulk foods > store. One might think that there would be a total shift to H&E as a routine > stain, especially with automated stainers becoming prevalent, but we have > successfully automated the stain on successive automated stainers. Since our > newest pathologists were trained as residents here, they are quite used to > HPS, and there appears to be little impetus to change. > > I still think the wafting of the boiling saffron is quite a pleasant aroma. > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 4 > Date: Fri, 6 Jan 2012 12:45:08 -0800 (PST) > From: Rene J Buesa <rjbu...@yahoo.com> > Subject: Re: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu>, Michele Carr > <michelecar...@yahoo.com> > Message-ID: > <1325882708.34952.yahoomailclas...@web65705.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > See attachment! > René J. > > --- On Fri, 1/6/12, Michele Carr <michelecar...@yahoo.com> wrote: > > > From: Michele Carr <michelecar...@yahoo.com> > Subject: [Histonet] finger nails > To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> > Date: Friday, January 6, 2012, 1:06 PM > > > Hi everyone was wondering what you do to get the nail from washing off the > slide during staining. The nail is extremely hard and seems to be washing > each time. Could I soften it prior to staining and what do you use to soften > it? Thanks in advance for all your responses. > Michele Carr > Medical Laboratory Services > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 5 > Date: Fri, 6 Jan 2012 17:06:19 -0500 > From: "Diana McCaig" <dmcc...@ckha.on.ca> > Subject: [Histonet] Quality assurance program for pathologists > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca> > Content-Type: text/plain; charset="us-ascii" > > Is anyone willing to share with me their quality assurance/management > program for pathologists. > > > > Sincere thanks > > Diana > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 9 > *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
