Re: Re: [Histonet] Re: IHC on frozens

2009-07-07 Thread TF
at section; this approach does not require HIER. 2009-07-07 TF 发件人: Colleen Forster 发送时间: 2009-07-07 02:41:59 收件人: Johnson, Teri 抄送: histonet@lists.utsouthwestern.edu 主题: Re: [Histonet] Re: IHC on frozens I disagree. I do heat retrieval on frozen sections that have been fixed in a fo

Re: [Histonet] Re: IHC on frozens

2009-07-06 Thread Colleen Forster
I disagree. I do heat retrieval on frozen sections that have been fixed in a formalin fix. If you use the correct protocol you can get very nice IHC on these. Colleen Forster HT(ASCP)QIHC Anatomic Pathology Research Laboratory U of MN Johnson, Teri wrote: In response to this thread: <

[Histonet] Re: IHC on frozens

2009-07-06 Thread Johnson, Teri
In response to this thread: >< Kimberly: Your question has a two parts answer: 1- it cannot be done because the sections will peel off, but most importantly 2- it is not necessary since HIER was developed to "undo" the cross linkage produced by the NBF fixation, and the tissues used for?FS are no