Bravo to Donna Emge for her detailed response on this issue. I am really
looking forward to seeing the YouTube videos you make. There seems to be a
critical lack of these available for histology technique!
Best wishes,
Teri Johnson
Manager, Histology
GNF - San Diego, CA
858-332-4752
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Hi Mohammad,
For the cracking during cryo sectioning of the mouse brain, that would likely
be a temperature issue. Make sure to give the samples just out of a -20 or -80
freezer 15 minutes or longer to adjust to the cryostat temperature before
sectioning. Fresh frozen brain typically requires a
