-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox
Sent: Monday, December 16, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Staining on Alcohol Fixed Smears
I agree that with no formalin exposure, there is no crosslinking that
needs to be broken, but a then, do
-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent: Tuesday, December 17, 2013 5:48 AM
To: 'Beth Cox'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Thanks
, December 17, 2013 5:48 AM
To: 'Beth Cox'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Thanks for the clarification. I appreciate the info!
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne
@lists.utsouthwestern.edu; levi.fr...@gmail.com
Subject: [Histonet] Re: Staining on Alcohol Fixed Smears
Levi,
I've worked up most of the more common antibodies on alcohol fixed
smears. There are two things to remember here: 1) your specimens are
alcohol fixed, meaning antigen retrieval needs
Dec 2013 11:13:59 -0500
From: Tom McNemartmcne...@lmhealth.org
Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears
To: 'Beth Cox'bethc...@gmail.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com
levi.fr...@gmail.com
Message-ID
Levi,
I've worked up most of the more common antibodies on alcohol fixed
smears. There are two things to remember here: 1) your specimens are
alcohol fixed, meaning antigen retrieval needs are different, and 2)
they are smears, meaning the cells weren't 'cut open' during microtomy,
which
