Nice! I may try one or both of your procedures (Adam & Andrea).
~Merced
--On Monday, September 07, 2009 3:09 PM -0400 Andrea Hooper
wrote:
I routinely stain with two primaries from same species. Mostly rat
antibodies on mouse tissue. I have been fortunate enough so far not to
have to do it
: SPAM-LOW: [Histonet] RE: Staining with two primary antibodies from
same host
Adam,Good point to prepare a protocol first, and then start staining! From
theoretical point of view your protocol should work. However, I have tried
this 'Jackson approach' using a Fab blocking step in a mous
How to label the antibody with FITC?
it is hard, or a complex procedure
-- Original --
From: "Andrea Hooper";
Date: Tue, Sep 8, 2009 03:09 AM
To: "Histonet";
Subject: [Histonet] RE: Staining with two primary antibodies f
I routinely stain with two primaries from same species. Mostly rat
antibodies on mouse tissue. I have been fortunate enough so far not
to have to do it in GFP containing tissue so I have utilized either
commercially available FITc labeled primaries, or we label them
ourselves. Then I use an ant
Well, a country pathologist like myself will never seen such a complex
staining routine, but I'd sure like to design a graphic for a lecture
about it!
I'd definitely start with the Bremen Town Musicians from Grimm -
http://www.elene-tlc.net/Bremen_Forum/Free_time_in_Bremen/The_Brementown_Musician
Adam,Good point to prepare a protocol first, and then start staining! From
theoretical point of view your protocol should work. However, I have tried this
'Jackson approach' using a Fab blocking step in a mouse-on-mouse situation
(before the MOM kits were available) without success. There are at
