To calibrate a pH meter, we use purchased pH buffers at pH 4.0, 7.0, and 10.0.
To perform the IHC method, we use TBS (pH 7.6) with Tween 20 as our rinse buffer. Jan Shivers On Thu, Jan 23, 2014 at 2:52 AM, Edwards, Richard <r...@leicester.ac.uk>wrote: > And what is the buffer of choice, PBS, TBS, with or without > protein/detergents or whatever, thanks. > > > Richard > Edwards > > > Leicester University > > > > U.K. > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers > Sent: 22 January 2014 22:08 > To: Laurie Colbert > Cc: Histonet Post (histonet@lists.utsouthwestern.edu) > Subject: Re: [Histonet] Buffer pH for IHC > > pH meter; Corning. > > > On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert <lcolb...@pathmdlabs.com > >wrote: > > > What does everyone use to test the pH of their buffers for IHC? Are > > pH strips adequate or do you have to have an actual pH meter? If you > > use a meter, any suggestions on a make and model? > > > > Laurie Colbert, HT (ASCP) > > Histology Supervisor > > PATH MD > > 8158 Beverly Blvd. > > Los Angeles, CA 90048 > > (323) 648-3214 direct > > (424) 245-7284 main lab > > > > The information contained in this transmission may contain privileged > > and confidential information, including patient information protected > > by federal and state privacy laws. It is intended only for the use of > > the > > person(s) named above. If you are not the intended recipient, you are > > hereby notified that any review, dissemination, distribution, or > > duplication of this communication is strictly prohibited. If you are > > not the intended recipient, please contact the sender by reply email > > and destroy all copies of the original message. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Jan Shivers > Senior Scientist > IHC/Histology Section Head > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet