As far as I remember enzymes are proteins and are they not supposed to
cross-link with formalin also?
René J.
--- On Mon, 9/29/08, Greg Dobbin <[EMAIL PROTECTED]> wrote:
From: Greg Dobbin <[EMAIL PROTECTED]>
Subject: Re: [Histonet] Re: Which one first - antigen retrieval or peroxida
Peroxidase is an enzyme. It doesn't miraculously appear as a result to
exposure to heat. You show me one one slide where this has occurred and
I will humbly remain silent in this forum for a whole month! Ok, maybe
just a week... :-)
Greg
Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
D
I'm with Rene on this one. Block after retrieval.
For those of you who do the block before retrieval, how do you control for
any activity that might be exposed during the retrieval step? Couldn't it
lead to false positives in your slides?
Albert C. Grobe, PhD
Tissue Engineering Lab
In
