Hi Bea,
Thank you for your response. The reason to 4% Glut use is just a PI
request (really a matter of preference), and it is only on tissue that
will not be immunostained. The ocular tissue I work with is rabbit. One
thing I noticed is that the tissue that is fixed for 24h takes longer to
Reilly; Bea DeBrosse-Serra; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue
Hi Bea,
Thank you for your response. The reason to 4% Glut use is just a PI request
(really a matter of preference), and it is only on tissue that will not be
immunostained
Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative
for eyes. With Davidson's you have two fast penetrating ingredients and one
slow acting fixative. Glut is only a fast fixative. Besides, the best
institutions who deal with eyes only use Davidson's. Just my thoughts.
