David,
The reason that I am able incubate overnight is that I antigen retrieve
my free-floating sections in the Biocare Decloaker (and no, I am not
receiving any subsidies from them). This has enabled me to reduce
incubation times as well as revealing receptor staining that has been
difficult to
Hi Histonet, Tina & Gil
I work on the same material as Tina and just wanted to echo
her "overnight" comments with the addition that I routinely
incubate 40um floating sections over the whole weekend at room
temp (on a very slow shaker, 20 rpm, and with azide to stop
bug growth. It all washes out b
