after Zinc fixation
what should you do before cutting sections on a cryostat/microtome?
need sucrose cryoprotection as well?
2009-10-22
TF
发件人： Melanie Black
发送时间： 2009-10-22 01:16:43
收件人： Patsy Ruegg
抄送： histonet
主题： [Histonet] Re: looking for CD31 for rat tissues
Hi Patsy
Yes
Hi Patsy
Yes it works very well. The antibody is cat # RDI-RTCD31-3A12 from
Fitzgerald. Yes it is zinc fixed. You use alk phos instead of DAB.
See pic.
Zinc Fixative (JB Fixative or ZSF)
0.1M Tris Buffer, pH 7.4
Tris Base 12.1 g (TRIZMA)
1N HCL
