Tyrone Genade asks: > I'm using the modified silver stain of Gallyas (J. Neurosci. Methods > 50:159--164, 1993) for microglia. It didn't work first time round so > I'm doing some trouble shooting. My main concern is the shelf life of > the potassium ferroscyanide solution (1 g K3Fe(CN)6 + 0.1 mL H2SO4 per > 100 mL). It is now a nice green color and looks a bit darker than > yesterday. Anyone know for how long this solution is good?
The spelling "ferroscyanide" doesn't exactly inspire confidence - do you mean "ferricyanide"? John Kiernan did a very nice review of Gallyas stains (of which there are apparently several) on Histonet about three years ago. See http://www.histosearch.com/histonet/Apr08A/Re.HistonetGallyasStain.html John Kiernan describes these methods as quite difficult. I have no experience with them. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet