Hi Patricia, I use Dako's neutrophil elastase clone NP57 (code MO752) on FFPE tissue sections. I couldn't get it to work until I did the peroxidase block after the application of the primary antibody - I stain with the neutrophil elastase for 1 hour, then apply H2O2 for 5 minutes (I use the ready to use one from Dako), then I use Dako's EnVision as my detection system for 30 minutes. I hope this helps!
> Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. need help with Elastase staining (Patricia F Lott) > 2. HPS stain (Gagnon, Eric) > 3. Re: Disinfecting Cryostat (Caroline Miller) > 4. AO 860 repairman in NC? (Caroline Bass) > 5. Re: HPS stain (Robert Richmond) > 6. special stain instrument (Santiago, Albert) > 7. thermowave users (godsgal...@aol.com) > 8. Techs from Rockford Health System (Steven Coakley) > 9. Microtomy of LEEP specimens (Joanne Clark) > 10. RE: Microtomy of LEEP specimens (Mike Pence) > 11. Re: Microtomy of LEEP specimens (Victor Tobias) > 12. RE: Microtomy of LEEP specimens (Laurie Colbert) > 13. cotton blue=acid blue=methyl blue (Edwards, R.E.) > 14. Breast tissue (rmweber...@comcast.net) > 15. NY DOH inspection question (Whitaker, Bonnie) > 16. Re: cotton blue=acid blue=methyl blue (Geoff McAuliffe) > 17. RE: NY DOH inspection question (McMahon, Loralee A) > 18. RE: Microtomy of LEEP specimens (Smith Wanda) > 19. Re: Breast tissue (Rene J Buesa) > 20. Ventana Ultra (thisis...@aol.com) > 21. spin columns (Emily Sours) > 22. spin columns (Pat Flannery) > 23. Re: Breast tissue (mari.ann.mailh...@leica-microsystems.com) > 24. RE: RE: Microtomy of LEEP specimens (Tom McNemar) > 25. Re: Breast tissue (Angela Bitting) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 9 Feb 2009 12:09:55 -0600 > From: "Patricia F Lott" <pl...@uab.edu> > Subject: [Histonet] need help with Elastase staining > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <dcfab2f06434294d8cf45c3e28c88d9e56c...@uabexmb8.ad.uab.edu> > Content-Type: text/plain; charset="US-ASCII" > > Does anyone have any experience with staining for Elastase in > Neutrophils in tissue sections? Any help would be appreciated! > > > > ------------------------------ > > Message: 2 > Date: Mon, 9 Feb 2009 13:11:50 -0500 > From: "Gagnon, Eric" <gagn...@kgh.kari.net> > Subject: [Histonet] HPS stain > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <f93bd6329fc3ae4c8db116b985fbc31327c3a...@kghmail.kgh.on.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Sharon, > > HPS is our routine stain (instead of H&E, which 98% of labs use). Not > sure what part of the stain you are having trouble with, but most > unpredictability usually comes in the phloxine/saffron balance. > > Some things to watch for are: > -keeping your saffron covered, and I would suggest parafilm-ing it when > not in use > -keep the alcohols before the saffron rigorously free of > phloxine-contaminated alcohol, thus not allowing tainted alcohol to > contaminate/dilute the saffron during staining > -getting all the background hematoxylin and phloxine out, which can be > checked as you go along when you validate your method > -be diligent about the preparation of the saffron. In addition to > Hermina's suggested method, we microwave the dry saffron, grind it, then > boil the alcoholic mixture to remove as much moisture as possible > > What do you use the stain for, i.e. differentiating muscle and collagen? > As a routine stain, I understand its use in Canada is limited to a few > laboratories in the east such as the Ottawa, Kingston, Sudbury areas. > This is often because pathologists who trained here at Queen's Pathology > like to take the stain with them. Having said that, many of our residents > request slides stained with H&E, to familiarize themselves with that stain > before exams. > > Good luck, hope this helps... > > Eric Gagnon MLT > Histology Laboratory, > Kingston General Hospital > Kingston, Ontario, Canada > > > > > ------------------------------ > > Message: 3 > Date: Mon, 9 Feb 2009 10:17:19 -0800 > From: Caroline Miller <cmil...@gladstone.ucsf.edu> > Subject: Re: [Histonet] Disinfecting Cryostat > To: lpw...@sbcglobal.net > Cc: histonet@lists.utsouthwestern.edu, 'Ingles Claire ' > <cing...@uwhealth.org> > Message-ID: <407e13f3-8499-4b03-bb56-e7dc95a94...@gladstone.ucsf.edu> > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes > > In the UK (where I used to run a clinical lab) we also would turn off > the cryostat in the morning, wait for it to defrost then just before > going home for the evening heat up some formalin in the microwave and > place that in the chamber and leave overnight. > > It certainly kills everything, but it is also kinda toxic to your > lungs! I wouldn't suggest it but it is a 'severe cleaning' option, > especially if you have had something nasty in there. > > This was about 5 years ago, the rules may have changed, and Yes, I do > realise just how bad that is for you and everyone around! > > Caroline > > > Caroline Miller > Co-Manager > J David Gladstone Institutes Histology and Microscopy Core > 1650 Owens St > San Francisco > CA 94158 > > http://www.gladstone.ucsf.edu/gladstone/site/histology/ > cmil...@gladstone.ucsf.edu > > > > > On Feb 7, 2009, at 1:11 PM, Lee & Peggy Wenk wrote: > >> 95-100% also work, for bacteria, fungus, viruses. They will not work >> on >> killing spores. That's where the 70% is to be used, as the water >> (30% part) >> is needed to soften the spore wall, so that the alcohol (70% part) >> can get >> inside. >> >> Peggy A. Wenk, HTL(ASCP)SLS >> Beaumont Hospital >> Royal Oak, MI 48073 >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles >> Claire >> Sent: Friday, February 06, 2009 6:38 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Disinfecting Cryostat >> >> Why no 95%? We have been using it in perpituity with no bad effects. >> The >> alcohol has evaporated from the inside surfaces by morning. >> >> Claire >> >> ________________________________ >> >> From: histonet-boun...@lists.utsouthwestern.edu on behalf of gayle >> callis >> Sent: Fri 2/6/2009 11:11 AM >> To: 'Louise Hartson'; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Disinfecting Cryostat >> >> >> >> We use 70% ethanol on gauze (just damp) to wipe down inside of >> cryostat. Do >> not use 95% or 100% alcohol, as 70% is the most effective for >> disinfection, >> commonly used in biohoods. We pick up little trimmings with first >> dampened >> gauze (or kim wipe) then re-wipe with a second gauze. We prefer >> gauze for >> its flexibility. Also, if you put a kimwipe or gauze behind knife >> holder to >> l catch trimmings then you can fold up kimwipe, and lift "garbage" >> out, >> intact, and go to biohazard container before wipe down. We try to >> avoid too >> much alcohol on knife holder parts since alcohol ruins lubricants >> needed for >> good operation. Check with your cryostat manufacturer to see what >> they >> recommend. It pays to wipe down under and over sliding glass door to >> counterattack biohazardous "cling-ons" found on your gloves, and where >> people have touched the instrument. 70% alcohol will not kill prions. >> >> Water based disinfectants freeze in cryostat. Eventually you need >> scheduled >> disinfection with cryostat in defrosted mode with approved >> disinfecting >> agent. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Mon, 09 Feb 2009 13:47:31 -0500 > From: Caroline Bass <cb...@wfubmc.edu> > Subject: [Histonet] AO 860 repairman in NC? > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <c5b5e473.3410%cb...@wfubmc.edu> > Content-Type: text/plain; charset="ISO-8859-1" > > Hello Everyone, > > I have refurbished an old AO 860 sliding microtome. I¹m pretty proud of > myself, I¹ve unlocked the totally gummed mechanism and have put it > together > myself. It seems to work very well. The only problem I have is tuning the > sliding block. I have no idea how to approach it, and after several failed > attempts (I can get it to slide down the length, but can¹t achieve a > smooth > movement), I¹m wondering if it¹s better to find a repairman in the area > that > can spend an hour showing me how to do this. > > Does anyone have suggestions? Do you know of any repairmen in the area? > > Thanks, > > Caroline Bass > > > ------------------------------ > > Message: 5 > Date: Mon, 9 Feb 2009 14:24:01 -0500 > From: Robert Richmond <rsrichm...@aol.com> > Subject: [Histonet] Re: HPS stain > To: histonet@lists.utsouthwestern.edu > Message-ID: > <abea52a60902091124h5d42780bg96bd654237f9c...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > The hematoxylin-phloxin- saffron stain, which supposedly originated at > the Montreal Neurological Institute, migrated from there to > Columbia-Presbyterian Hospital in New York City, where it was used as > the general oversight stain in surgical pathology at least into the > 1960's. When I saw it in use there in 1966 they didn't seem to be > having trouble with it. > > On the other side of Central Park, New York Hospital (Cornell Medical > Center) had used a Light Green trichrome stain as a general oversight > stain. I think they abandoned it in favor of H & E after Chandler > Foot's retirement in 1948. > > Saffron is a natural dye derived from the stigmas of the saffron > crocus, Crocus sativus. Although it was (and is) extremely expensive, > it was used both as a textile dye and as a spice (in paella, for > example). It's sometimes seen (and smelled) as a component of > expensive curry powders - my wife and I have been experimenting with > Penzey's Maharajah curry powder, which is 2% saffron. The immense hand > labor of extracting all those stigmas from the flowers makes saffron > "the most expensive spice in the world". > > Saffron as a histologic dye has traditionally been specified as > "safran du Gâtinais" - from a particular region of France - though I > am not certain that such a product still actually exists. I would > think that any good quality saffron would suffice (don't substitute > safflower, 'dyer's saffron'). You'll pay at least ten dollars a gram > for saffron, at Penzeys anyway. > > Some recipes specified as many as seven changes of hot alcohol to > extract the dye, and some people used a reflux condenser for the > extraction. The alcohol extract has a strong medicinal smell which > some people find quite unpleasant. > > (I have no connection with Penzeys.com, except that my wife and I have > gotten addicted to their shipments of very high quality spices.) > > Bob Richmond > Samurai Pathologist > Knoxville TN > > > > ------------------------------ > > Message: 6 > Date: Mon, 9 Feb 2009 14:57:24 -0500 > From: "Santiago, Albert" <albert.santi...@uphs.upenn.edu> > Subject: [Histonet] special stain instrument > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <bb0f4d2041eed9458ad95a538dbc4cae02fa7...@uphsmbx6.uphs.pennhealth.prv> > > Content-Type: text/plain; charset="us-ascii" > > Hello again fellow histonetters, I have an old artisan special stain/ihc > stain stainer that I'm looking to replace with a newer model, but before > I commit to it I was wondering if anyone else uses any other special > stain instrument that I can look into and compare.... Thanks for your > help.... > > > > Albert Santiago, HT (ASCP) > > Laboratory Supervisor > > Dermatopathology > > Phone 215-662-6539 > > Fax 215-662-6150 > > > > > > > > > > The information contained in this e-mail message is intended only for the > personal and confidential use of the recipient(s) named above. If the > reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > > ------------------------------ > > Message: 7 > Date: Mon, 09 Feb 2009 15:03:47 -0500 > From: godsgal...@aol.com > Subject: [Histonet] thermowave users > To: histo...@pathology.swmed.edu > Message-ID: <8cb5908a749d8f9-f58-...@webmail-da13.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > Can any ThermoWave users that microwave prostates please email me offline, > if you are willing to share SOPs.... > > > Thanks, > > Roxanne > > > ------------------------------ > > Message: 8 > Date: Mon, 9 Feb 2009 12:04:49 -0800 (PST) > From: Steven Coakley <sjchta...@yahoo.com> > Subject: [Histonet] Techs from Rockford Health System > To: Histonet@lists.utsouthwestern.edu > Message-ID: <219757.8126...@web38205.mail.mud.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Wondering if there are any tech here from Rockford Health System, > Rockford, Ill. > > > > > ------------------------------ > > Message: 9 > Date: Mon, 9 Feb 2009 13:39:11 -0700 > From: "Joanne Clark" <jcl...@pcnm.com> > Subject: [Histonet] Microtomy of LEEP specimens > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <0cda5e1e01301f4880a8a7a8bcbda39cf66...@mail.pcnm.com> > Content-Type: text/plain; charset="us-ascii" > > Hi, > > > > How many of you have a protocol to cut multiple deepers/levels on LEEP > specimens and if so, how many do you cut? > > > > Thanks > > Joanne Clark, HT, MLT > > Pathology Consultants of New Mexico > > Roswell, NM > > > > ------------------------------ > > Message: 10 > Date: Mon, 9 Feb 2009 14:59:33 -0600 > From: "Mike Pence" <mpe...@grhs.net> > Subject: RE: [Histonet] Microtomy of LEEP specimens > To: "Joanne Clark" <jcl...@pcnm.com>, > <histonet@lists.utsouthwestern.edu> > Message-ID: > <661949901a768e4f9cc16d8af8f2838c017a3...@is-e2k3.grhs.net> > Content-Type: text/plain; charset="us-ascii" > > We cut 3 levels on each block. > Mike > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne > Clark > Sent: Monday, February 09, 2009 2:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtomy of LEEP specimens > > > Hi, > > > > How many of you have a protocol to cut multiple deepers/levels on LEEP > specimens and if so, how many do you cut? > > > > Thanks > > Joanne Clark, HT, MLT > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 11 > Date: Mon, 09 Feb 2009 14:08:14 -0800 > From: Victor Tobias <vic...@pathology.washington.edu> > Subject: Re: [Histonet] Microtomy of LEEP specimens > To: Joanne Clark <jcl...@pcnm.com> > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <4990a94e.9030...@pathology.washington.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > 3 levels. > > Victor Tobias > Clinical Applications Analyst > University of Washington Medical Center > Dept of Pathology Room BB220 > 1959 NE Pacific > Seattle, WA 98195 > vic...@pathology.washington.edu > 206-598-2792 > 206-598-7659 Fax > ================================================= > Privileged, confidential or patient identifiable information may be > contained in this message. This information is meant only for the use > of the intended recipients. If you are not the intended recipient, or > if the message has been addressed to you in error, do not read, > disclose, reproduce, distribute, disseminate or otherwise use this > transmission. Instead, please notify the sender by reply e-mail, and > then destroy all copies of the message and any attachments. > > > > Joanne Clark wrote: >> Hi, >> >> >> >> How many of you have a protocol to cut multiple deepers/levels on LEEP >> specimens and if so, how many do you cut? >> >> >> >> Thanks >> >> Joanne Clark, HT, MLT >> >> Pathology Consultants of New Mexico >> >> Roswell, NM >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > ------------------------------ > > Message: 12 > Date: Mon, 9 Feb 2009 14:10:46 -0800 > From: "Laurie Colbert" <laurie.colb...@huntingtonhospital.com> > Subject: RE: [Histonet] Microtomy of LEEP specimens > To: "Joanne Clark" <jcl...@pcnm.com>, > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <57be698966d5c54eae8612e8941d768304e9d...@exchange3.huntingtonhospital.com> > > Content-Type: text/plain; charset="us-ascii" > > Three levels on all blocks > > Laurie Colbert > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne > Clark > Sent: Monday, February 09, 2009 12:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtomy of LEEP specimens > > Hi, > > > > How many of you have a protocol to cut multiple deepers/levels on LEEP > specimens and if so, how many do you cut? > > > > Thanks > > Joanne Clark, HT, MLT > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 13 > Date: Tue, 10 Feb 2009 11:45:11 +0000 > From: "Edwards, R.E." <r...@leicester.ac.uk> > Subject: [Histonet] cotton blue=acid blue=methyl blue > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <7722595275a4dd4fa225b92cdbf174a1745423d...@exc-mbx3.cfs.le.ac.uk> > Content-Type: text/plain; charset="us-ascii" > > > Looking for a supplier please, I am aware that Sigma/Fluka supply a > ready made solution, which apparently does not work. > > Many thanks > > Richard Edwards > Leicester University > > U.K. > > > > > > ------------------------------ > > Message: 14 > Date: Tue, 10 Feb 2009 13:49:58 +0000 (UTC) > From: rmweber...@comcast.net > Subject: [Histonet] Breast tissue > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <1347926049.554711234273798846.javamail.r...@sz0046a.westchester.pa.mail.comcast.net> > > Content-Type: text/plain; charset=utf-8 > > > > > Hello,   Do anyone have a better remedy to process fatty breast tissue > other than after processing pressing it in paper towels and then putting > it back in paraffin for a couple of hours.  The pathologist are saying > this is taking to long. > > > > Thanks, > > > > > > > ------------------------------ > > Message: 15 > Date: Tue, 10 Feb 2009 09:26:02 -0500 > From: "Whitaker, Bonnie" <bonnie.whita...@osumc.edu> > Subject: [Histonet] NY DOH inspection question > To: histo...@pathology.swmed.edu > Message-ID: <3ce20ed86c4a114ebdf3bce8defd8f60801...@msxc06.osumc.edu> > Content-Type: text/plain; charset=us-ascii > > Hi All, > > Will some of you that are inspected by the NYDOH please email me with how > you > meet the requirement (below) for performance verification for alcohol, > xylene > and formalin? > Clinical Laboratory Standards of Practice, Part 1- General Systems; > Reagents > Sustaining Standard of Practice 4 (REAG S4): Inventory Control. > The standard states that the inventory log should include the following > information: lot numbers, expiration dates, the date of receipt in the > laboratory, date of performance verification and the date the material is > placed in service, it is a requirement. > > > Thanks!! > > Bonnie Whitaker > Clinical Histology Manager > Ohio State University Medical Center > 614.293.5048 > > > > ------------------------------ > > Message: 16 > Date: Tue, 10 Feb 2009 09:55:10 -0500 > From: Geoff McAuliffe <mcaul...@umdnj.edu> > Subject: Re: [Histonet] cotton blue=acid blue=methyl blue > To: "Edwards, R.E." <r...@leicester.ac.uk> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <4991954e.7020...@umdnj.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Sounds like you want Aniline Blue, CI 42780. Much easier to find by that > name. > > Geoff > > Edwards, R.E. wrote: >> Looking for a supplier please, I am aware that Sigma/Fluka supply >> a ready made solution, which apparently does not work. >> >> Many thanks >> >> Richard Edwards >> Leicester University >> >> U.K. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583 > mcaul...@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 17 > Date: Tue, 10 Feb 2009 09:58:43 -0500 > From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> > Subject: RE: [Histonet] NY DOH inspection question > To: "Whitaker, Bonnie" <bonnie.whita...@osumc.edu>, > <histo...@pathology.swmed.edu> > Message-ID: > <2cf6f6b05263ea4ebab07781b51e5db002c94...@e2k3ms1.urmc-sh.rochester.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > I would be interested in how you verify the performance of the xylenes and > alcohols as well. Thank you in advance. > > Loralee McMahon, HTL (ASCP) > ICC Supervisor > University of Rochester > Department of Pathology > > (585) 275-7210 > > > ________________________________ > > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Whitaker, > Bonnie > Sent: Tue 2/10/2009 9:26 AM > To: histo...@pathology.swmed.edu > Subject: [Histonet] NY DOH inspection question > > > > Hi All, > > Will some of you that are inspected by the NYDOH please email me with how > you > meet the requirement (below) for performance verification for alcohol, > xylene > and formalin? > Clinical Laboratory Standards of Practice, Part 1- General Systems; > Reagents > Sustaining Standard of Practice 4 (REAG S4): Inventory Control. > The standard states that the inventory log should include the following > information: lot numbers, expiration dates, the date of receipt in the > laboratory, date of performance verification and the date the material is > placed in service, it is a requirement. > > > Thanks!! > > Bonnie Whitaker > Clinical Histology Manager > Ohio State University Medical Center > 614.293.5048 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 18 > Date: Tue, 10 Feb 2009 09:06:02 -0600 > From: Smith Wanda <wanda.sm...@hcahealthcare.com> > Subject: [Histonet] RE: Microtomy of LEEP specimens > To: Joanne Clark <jcl...@pcnm.com>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <9e2d36ce2d7cba4a94d9b22e8328a3bacbe95...@nadcwpmsgcms03.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > We routinely cut 3 levels on 3 slides for each block. > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne > Clark > Sent: Monday, February 09, 2009 3:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtomy of LEEP specimens > > Hi, > > > > How many of you have a protocol to cut multiple deepers/levels on LEEP > specimens and if so, how many do you cut? > > > > Thanks > > Joanne Clark, HT, MLT > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 19 > Date: Tue, 10 Feb 2009 07:21:09 -0800 (PST) > From: Rene J Buesa <rjbu...@yahoo.com> > Subject: Re: [Histonet] Breast tissue > To: histonet@lists.utsouthwestern.edu, rmweber...@comcast.net > Message-ID: <636989.62353...@web65701.mail.ac4.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > The "better remedy" is to cut the slices thin and fix/process correctly, > specially using mineral oil. > René J. > > --- On Tue, 2/10/09, rmweber...@comcast.net <rmweber...@comcast.net> > wrote: > > From: rmweber...@comcast.net <rmweber...@comcast.net> > Subject: [Histonet] Breast tissue > To: histonet@lists.utsouthwestern.edu > Date: Tuesday, February 10, 2009, 8:49 AM > > > Hello, Do anyone have a better remedy to process fatty breast tissue > other than after processing pressing it in paper towels and then putting > it back > in paraffin for a couple of hours. The pathologist are saying this is > taking > to long. > > > > Thanks, > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 20 > Date: Tue, 10 Feb 2009 10:34:13 -0500 > From: thisis...@aol.com > Subject: [Histonet] Ventana Ultra > To: histonet@lists.utsouthwestern.edu > Cc: ali.ma...@ventana.roche.com > Message-ID: <8cb59ac28eaadee-708...@mblk-d24.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > I am considering purchasing a Ventana Benchmark Ultra IHC.? Does anyone > have any feedback concerning this piece of equipment.? Any feedback would > be appreciated. > Thanks, > Ann > > > ------------------------------ > > Message: 21 > Date: Tue, 10 Feb 2009 10:36:56 -0500 > From: Emily Sours <talulahg...@gmail.com> > Subject: [Histonet] spin columns > To: histonet@lists.utsouthwestern.edu > Message-ID: > <b39794b0902100736k767f8bb6v827cff813efdd...@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Hello > > Anyone know where to purchase QIAquick spin columns? If you get them from > Qiagen, they aren't sold separately, you have to buy them in a complete > kit. I have a feeling I'm stuck doing so. > > Emily > -- > It's like hearing Billy Joel play "Piano Man"-- joyless for all involved, > but demanded by a higher power. > --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax > > > ------------------------------ > > Message: 22 > Date: Tue, 10 Feb 2009 10:58:22 -0500 > From: Pat Flannery <pjfne...@duke.edu> > Subject: [Histonet] spin columns > To: histonet@lists.utsouthwestern.edu > Message-ID: <64d46715-499c-48b9-8cfa-edbebf95b...@duke.edu> > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes > > Emily- > > I just got a mailing from Genesee Scientific (800-789-5550, Ext. 2) > for their UniPrep columns. They're selling them specially for those > people who always run out of columns before they run out of reagents. > I think there are a couple of types, so you might want to contact them > to see which one you'd need. > > Hope this helps. > > -Pat Flannery > Duke Med Center > > >> Hello >> >> Anyone know where to purchase QIAquick spin columns? If you get them >> from >> Qiagen, they aren't sold separately, you have to buy them in a >> complete >> kit. I have a feeling I'm stuck doing so. >> >> Emily >> -- >> It's like hearing Billy Joel play "Piano Man"-- joyless for all >> involved, >> but demanded by a higher power. >> --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax >> _______________________________________________ > > > > ------------------------------ > > Message: 23 > Date: Tue, 10 Feb 2009 10:10:53 -0600 > From: mari.ann.mailh...@leica-microsystems.com > Subject: Re: [Histonet] Breast tissue > To: rmweber...@comcast.net > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > > <offd3c1dad.63e32cca-on86257559.0057b91e-86257559.00592...@leica-microsystems.com> > > Content-Type: text/plain; charset=ISO-8859-1 > > As long as you smell the clearant in the block then putting the blocks in > paraffin longer works. I never found it necessary to roll the fat in paper > towel and place the specimen back in paraffin. If the block is not fixed > ore dehydrated properly and cleared properly putting them in paraffin > doesn't work. > > It all starts at the gross station. Cutting nice thin sections that are > fixed for at least 24 hrs. Even before that breast should be bread loafed > and placed in formalin and fixed for 24 hrs. Then a section is cut and > placed in a cassette and that sits in formalin until it gets placed on the > processor. > > The process of rolling fatty tissue in paper towel and gently pressed > allows the fatty specimen to go back in formalin and be reprocessed > without > taking the specimen back through the xylenes and alcohols to reprocess. > > Hope this helps. > > Best regards > > Mari Ann Mailhiot BA HT ASCP > Application Specialist/Trainer > Leica Microsystems > Biosystems Division > Technical Assistance Center > 800 248 0123 x7267 > 847 236 3063 fax > mari.ann.mailh...@leica-microsystems.com > www.leica-microsystems.com > > > > rmweber...@comcas > t.net > Sent by: To > histonet-bounces@ histonet@lists.utsouthwestern.edu > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Breast tissue > 02/10/2009 07:49 > AM > > > > > > > > > > > Hello, Do anyone have a better remedy to process fatty breast tissue > other than after processing pressing it in paper towels and then putting > it > back in paraffin for a couple of hours. The pathologist are saying this > is taking to long. > > > > Thanks, > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > > > ------------------------------ > > Message: 24 > Date: Tue, 10 Feb 2009 11:36:31 -0500 > From: "Tom McNemar" <tmcne...@lmhealth.org> > Subject: RE: [Histonet] RE: Microtomy of LEEP specimens > To: "Smith Wanda" <wanda.sm...@hcahealthcare.com>, "Joanne Clark" > <jcl...@pcnm.com>, <histonet@lists.utsouthwestern.edu> > Message-ID: > <51d5d78fbedaea4fbccd9a9d44211dc505b8e...@lmhsmail.lmhealth.org> > Content-Type: text/plain; charset="iso-8859-1" > > Same here. > > Tom McNemar, HT(ASCP) > Histology Co-ordinator > Licking Memorial Health Systems > (740) 348-4163 > (740) 348-4166 > tmcne...@lmhealth.org > www.LMHealth.org > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Smith > Wanda > Sent: Tuesday, February 10, 2009 10:06 AM > To: Joanne Clark; histonet@lists.utsouthwestern.edu > Subject: [Histonet] RE: Microtomy of LEEP specimens > > > We routinely cut 3 levels on 3 slides for each block. > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne > Clark > Sent: Monday, February 09, 2009 3:39 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Microtomy of LEEP specimens > > Hi, > > > > How many of you have a protocol to cut multiple deepers/levels on LEEP > specimens and if so, how many do you cut? > > > > Thanks > > Joanne Clark, HT, MLT > > Pathology Consultants of New Mexico > > Roswell, NM > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 25 > Date: Tue, 10 Feb 2009 12:09:45 -0500 > From: "Angela Bitting" <akbitt...@geisinger.edu> > Subject: Re: [Histonet] Breast tissue > To: <rmweber...@comcast.net>, > <mari.ann.mailh...@leica-microsystems.com> > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <49916e89.2b7f.00c...@geisinger.edu> > Content-Type: text/plain; charset=US-ASCII > > We extended our paraffin infiltration times and that worked out well for > us too. > >>>> <mari.ann.mailh...@leica-microsystems.com> 2/10/2009 11:10 AM >>> > As long as you smell the clearant in the block then putting the blocks in > paraffin longer works. I never found it necessary to roll the fat in paper > towel and place the specimen back in paraffin. If the block is not fixed > ore dehydrated properly and cleared properly putting them in paraffin > doesn't work. > > It all starts at the gross station. Cutting nice thin sections that are > fixed for at least 24 hrs. Even before that breast should be bread loafed > and placed in formalin and fixed for 24 hrs. Then a section is cut and > placed in a cassette and that sits in formalin until it gets placed on the > processor. > > The process of rolling fatty tissue in paper towel and gently pressed > allows the fatty specimen to go back in formalin and be reprocessed > without > taking the specimen back through the xylenes and alcohols to reprocess. > > Hope this helps. > > Best regards > > Mari Ann Mailhiot BA HT ASCP > Application Specialist/Trainer > Leica Microsystems > Biosystems Division > Technical Assistance Center > 800 248 0123 x7267 > 847 236 3063 fax > mari.ann.mailh...@leica-microsystems.com > www.leica-microsystems.com > > > > rmweber...@comcas > t.net > Sent by: To > histonet-bounces@ histonet@lists.utsouthwestern.edu > lists.utsouthwest cc > ern.edu > Subject > [Histonet] Breast tissue > 02/10/2009 07:49 > AM > > > > > > > > > > > Hello, Do anyone have a better remedy to process fatty breast tissue > other than after processing pressing it in paper towels and then putting > it > back in paraffin for a couple of hours. The pathologist are saying this > is taking to long. > > > > Thanks, > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ______________________________________________________________________ > This email has been scanned by the MessageLabs Email Security System. > For more information please visit http://www.messagelabs.com/email > ______________________________________________________________________ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It > is intended solely for the addressee. Access to this message by anyone > else is unauthorized. If you are not the intended recipient, any > disclosure, copying, distribution or any action taken, or omitted to be > taken, in reliance on it is prohibited and may be unlawful. If you have > received this message in error, please delete all electronic copies of > this message (and the documents attached to it, if any), destroy any hard > copies you may have created and notify me immediately by replying to this > email. Thank you. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 63, Issue 12 > **************************************** > Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145 02 98455775 or 0408119276 sarah_tar...@wmi.usyd.edu.au _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet