Why are you fixing ? Try unfixed frozen? Or, why not Pwax embedded? They cut well in Pwax. Sure, you may have to "polish" the cut surface with tissue damped in water/20% alcohol to prevent cracking/curling just before each section is cut.
Or, after fixation, place in 30% sucrose until kidneys sink. Store at 4C until you freeze ( if not immediately). You will have to try several approaches until you find the correct one for your Lab. Good luck. Carl Hobbs Histology and Imaging Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet