Re: [Histonet] Re guinea pig IHC

2019-02-03 Thread Patsy Ruegg via Histonet
PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re guinea pig IHC Ms Ruegg, as usual , gives excellent advice: avoid HRP. Use Alk phos? Block end. alk phos by using levamisole. However, Ms Shivers does not state the fixation status of her FS. Is the Gpig tissue perfused-fixed

[Histonet] Re guinea pig IHC

2019-02-02 Thread Hobbs, Carl via Histonet
Ms Ruegg, as usual , gives excellent advice: avoid HRP. Use Alk phos? Block end. alk phos by using levamisole. However, Ms Shivers does not state the fixation status of her FS. Is the Gpig tissue perfused-fixed then frozen orfrozen as unfixed...then fixed? Alsowhy 0.3% H2O2? Use