Hello Histonetters, I have been staining residues with Phloroglucinol which is light sensitive however I due to time constraints I have not been able to wait for all the colour to fade ( as this takes about one week). Therefore, I have dried off the stained residue under lights and then counterstained with Toluidine Blue. This seems to be ok, with the Toluidine Blue restaining the residue as expected. I was wondering if anyone knows of any possible interaction with Phloroglucinol (in HCL) and Toluidine Blue ? Likewise the residue stained with Phloroglucinol and dried seems to develop almost crystal like tracks that disappear when restained. I am not sure if the "tracks" are due to Phloroglucinol reacting with light? Any ideas welcomed.
Thank you Birgitta Stephenson Microscopy Research Lab, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm -- http://www.fastmail.fm - The way an email service should be _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
