Re: [Histonet] Retic staining

container. Debbie Siena Get Outlook for iOS<https://aka.ms/o0ukef> From: Cassie P. Davis via Histonet Sent: Tuesday, April 23, 2019 1:07 PM To: histonet Subject: [Histonet] Retic staining *** Externally sourced email message *** Hi Charles, in addit

Re: [Histonet] Retic staining

Hi Charles, Ammoniacal Silver is a tricky step, we've had a lot of repeat Bielschowsky stains in the past due to it. You don't need to go drop by drop, what's key is to gently stir the solution as you slowly add ammonium hydroxide until the precipitate is reincorporated back into the solution.

[Histonet] Retic staining

Hi Charles, in addition to the drop size clean glassware makes a big difference. I used to use new urine cups to ensure the silver was being made in a clean vessel. I hope this helps Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org

[Histonet] Retic staining

I am having trouble getting my retic stain to come out strong. It is working but Pathologists are saying they shouldn't have to go to high power just to see it (and I agree). I am using a brand new American MasterTech Chandler's Precision kit. I have followed all the steps correctly (one difficult