Rather than use Halt (or other adhesive) in your water bath, have you tried using charged slides - those used for IHC. Then air-dry or heat dry the tissues before deparaffinizing. Any water left in the tissue will cause wash-offs. Do not lower the pH of the silver solution.
Cheryl Crowder, BA, HTL(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
