Re: [Histonet] Running ISH on the Ventana Ultra Stainers

2017-09-20 Thread Eddie Martin via Histonet
Hi Miranda, For starters, perform a Decontamination on your Ultras, don't dilute your probes. At our lab, we manually dispense the probes but a prep kit is fine. And also use a toothpick to clear out any gunk in your detection dispensers prior to doing a run. Also, check if your ultras are on

[Histonet] Running ISH on the Ventana Ultra Stainers

2017-09-19 Thread Miranda Giorgi via Histonet
Hello Histonetters, Our lab is in the process of trying to validate Kappa, Lambda and EBER ISH on the Ventana Ultra stainers. We have run these probes on the XT in the past with good results, but unfortunately staining on the Ultras is either poor or non-existent. We have tried several