Hello all -
Is there a more effective and faster way to remove the film from the slide?
I had to decolorize a couple of HE slides to do special stains, and it took
5 days for the film to remove. The slides were only a few days old and I
soaked it in fresh xylene. I almost removed it too soon
...@clinlab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Paula
Lucas
Sent: Thursday, July 29, 2010 1:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sakura Film Removal
Hello all
AnnieinArabia
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-Original Message-
From: Paula Lucas plu...@biopath.org
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Thu, 29 Jul 2010 12:17:54
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Sakura
We use a 50-50 solution of xylene and acetone. Depending on how long the
coverslips have been on, it takes from 20 minutes to an hour for them to come
off. If you are not decolorizing and re-staining, don't leave them too long in
the solution as the stain will start to come out.
Sharon E.
Lucas; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sakura Film Removal
We use a 50-50 solution of xylene and acetone. Depending on how long the
coverslips have been on, it takes from 20 minutes to an hour for them to come
off. If you are not decolorizing and re-staining, don't