[Histonet] Sakura Film Removal

2010-07-29 Thread Paula Lucas
Hello all - Is there a more effective and faster way to remove the film from the slide? I had to decolorize a couple of HE slides to do special stains, and it took 5 days for the film to remove. The slides were only a few days old and I soaked it in fresh xylene. I almost removed it too soon

RE: [Histonet] Sakura Film Removal

2010-07-29 Thread Jason McGough
...@clinlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Paula Lucas Sent: Thursday, July 29, 2010 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura Film Removal Hello all

Re: [Histonet] Sakura Film Removal

2010-07-29 Thread annigyg
AnnieinArabia Empower your Business with BlackBerry® and Mobile Solutions from Etisalat -Original Message- From: Paula Lucas plu...@biopath.org Sender: histonet-boun...@lists.utsouthwestern.edu Date: Thu, 29 Jul 2010 12:17:54 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura

Re: [Histonet] Sakura Film Removal

2010-07-29 Thread Sharon Scalise
We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't leave them too long in the solution as the stain will start to come out. Sharon E.

RE: [Histonet] Sakura Film Removal

2010-07-29 Thread Mighnon Lashus
Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Film Removal We use a 50-50 solution of xylene and acetone. Depending on how long the coverslips have been on, it takes from 20 minutes to an hour for them to come off. If you are not decolorizing and re-staining, don't