Hi Greg,
Permit me a few thoughts on factors not mentioned in your email:
* Do your slides 'look' baked/is the wax melted when they are removed from
the oven?
* Also, is there variance in your oven temperature?
* Especially vexing is the fact that control sections are lifting. Are
hwestern.edu
Subject: RE: [Histonet] Section adherence issues (IHC)
We have had really good luck with both the TOMO and Millennia 2000 adhesion
slides. We initially tried using the TOMO slides for everything (IHC, special
stains, and H's), but found that there was too much background staining on
Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 12, 2017 11:09 AM
To: histonet@lists.utsouthwestern.edu
Cc: kgevele...@ihis.org
Subject: [Histonet] Section adherence issues (IHC)
Hi Folks,
I haven't been on here much lately but it is nice to know that we are all here
Hi Folks,
I haven't been on here much lately but it is nice to know that we are all
here for each other when needed!
My problem is section adherence during IHC staining. And it is not all of
the time it is intermittent; so not all of the time and not on all slides
when it does occur.
Background:
