hi mia!
yes, you do need picric acid. the function of it is to solubilize all low
molecular weight proteins that would interefe with the sirius red stain. this
old collagen stain function very well and it is selective for collagen when you
use bouin fixative. if not, it is not selective. then if
Hi Mia,
I tried few different sources of Picric acid. Unfortunately, not all of them
worked. The one that is working is from Rowley Biochemical Institute -
www.rowleybio.com . I got a small bottle to try - 4 oz. Cat # SO-128. You
can probably find it from one of the large vendors.
Just a h
oun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia
> Woodruff
> Sent: Tuesday, February 22, 2011 10:33 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sirius red stain - picric acid substitute - water
> -really?
>
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Sirius red stain - picric acid substitute - water
-really?
I would do a trial run on "extra" slides or non-valuable tissue?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:his
estern.edu
Subject: [Histonet] Sirius red stain - picric acid substitute - water -really?
Hello all,
I have been undertaking sirius red staining using picric acid, I was under the
impression (from papers) that picric acid is an important component of the
procedure and read that it prevents no
Hello all,
I have been undertaking sirius red staining using picric acid, I was under the
impression (from papers) that picric acid is an important component of the
procedure and read that it prevents non-specific binding of the dye to things
other than collagen. However, I have recently found
