Our SMAD-4 is run on the Biocare Intellipath using the Santa Cruz B-8
clone. We do Diva retrieval, 1:200 dilution for 30 minutes followed
by PeroxAbolish to eliminate non-specific background staining. Our
pathology group has been happy with this.
On Tue, Mar 6, 2012 at 5:34 AM, Angela Bitting wr
I've tried to work it up on the XT also with inconsistent, weak results. If you
get a reply on this one, let me know.
Angie
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5
Hi,
I was wondering if anyone is running IHC for Smad4 (B-8) / DPC4. We are
currently trying to work up this antibody for validation; however, we are
having significant problems getting it to work consistently. We are using the
Ventana Benchmark XT. The antibody is from Santa Cruz Biotechnology
