Re: [Histonet] Stain line order

2021-10-26 Thread Mac Donald, Jennifer via Histonet
? -Original Message- From: Anne Murvosh via Histonet Sent: Tuesday, October 26, 2021 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stain line order EXTERNAL SENDER- Exercise caution with requests, links, and attachments. When doing frozen section staining for Mohs we have DI

[Histonet] Stain line order

2021-10-26 Thread Anne Murvosh via Histonet
When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing,