We fix our embryos overnight in 4% para for in situ's--the long crosslinking
helps fix the RNA better. For some immuno's though, too much fixing is
definitely bad. Why not store in PBS until you're ready to dehydrate?
Oh, (a note of caution?), we don't use para fixation for paraffin embedding,
th
Dear all,
I am doing some experiments where mouse skin tissue is harvested for
immuno and in situ staining at 2, 4, 6 and 8 days following a treatment.
The usual protocol used in our lab (for other tissues, usually bone) is to
fix overnight in 4% PFA before dehydration and paraffin embedding. As
