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From: prueg...@hotmail.com<mailto:prueg...@hotmail.com>
To: wa...@livemail.uthscsa.edu<mailto:wa...@livemail.uthscsa.edu>
Subject: RE: [Histonet] TRAP staining protocol troubleshooting
Date: Mon, 17 Nov 2014 13:22:24 -0700
Trap dose not work on decalcified bone unless you have dec
: [Histonet] TRAP staining protocol troubleshooting
Date: Mon, 17 Nov 2014 13:22:24 -0700
Trap dose not work on decalcified bone unless you have decaled with EDTA in my
experience.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
Hello histonetter heroes! I have an issue with a TRAP staining protocol that
I'm currently using that involves Fast Red Violet LB Salt for the staining of
the Osteoclast and then Fast Green as a counterstain. The osteoclast are
suppose to stain Red Violet and the background is suppose to stain
herrick.ja...@mayo.edu> To:
> histonet@lists.utsouthwestern.edu> Subject: [Histonet] TRAP Staining
> Protocol> > Hello everyone,> > I have a question regarding a good TRAP stain
> protocol. We have pretty good luck using our protocol on human iliac crest
> biopsi
Hello everyone,
I have a question regarding a good TRAP stain protocol. We have pretty good
luck using our protocol on human iliac crest biopsies, but the stain tends to
fade to almost nothing overnight, when we use it on rat and mouse femurs,
vertebrae, etc.. We do see some fading in our iliac
